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Primary Antibodies  >  DNA Damage and Repair Antibodies
 

EIF3F Antibody

Rabbit Polyclonal
Code

600-401-934
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western blot using Rockland's affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2).  Cdk11 is responsible for phosphorylating eIF3f in vivo.  After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200.  This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
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Synonyms: eIF 3 epsilon antibody, eIF3 p47 antibody, eIF3 p47 subunit antibody, EIF3S5 antibody, Eukaryotic translation initiation factor 3 subunit 5 antibody

EIF3F Antibody Properties

Description
Anti-eIF3f (RABBIT) Antibody - 600-401-934
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, chimpanzee, chicken, bovine
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
Western Blot : 1:200 - 1:2,000
Immunochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
EIF3F
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Lot#  

EIF3F Antibody Description

Background
eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 114-125 of human eIF3f protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against human eIF3f protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with eIF3f protein from human, mouse, rat, chimpanzee, chicken, and bovine based on 100% homology with the immunizing sequence.  Partial reactivity is expected against eIF3f from Drosophila melanogaster and Tetraodon nigroviridis (pufferfish) based on 90% homology of the immunizing sequence with eIF3f from these sources.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, chimpanzee
Catalog Number
600-401-494

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse, human, rat, dog, bovine, monkey
Catalog Number
600-401-A62

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry