House Phospho Banner
 
You may also want to consider these related products.
Anti-EGFR (RABBIT) Antibody - 100-401-149
Anti-EGFR (RABBIT) Antibody - 100-401-149
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-EGFR (RABBIT) Antibody - 600-401-905
Anti-EGFR (RABBIT) Antibody - 600-401-905
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Primary Antibodies  >  Phospho Specific Antibodies
 

EGFR phospho Y1197 Antibody

Rabbit Polyclonal
Code

600-401-928
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Immunohistochemistry of Rabbit anti-EGFR pY1197 antibody.  
Tissue:  placental trophoblasts.  
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  EGFR pY1197 antibody  at 5 µg/ml for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization: EGFR pY1197 is on the cell membrane.
Staining:  EGFR pY1197 as precipitated red signal with hematoxylin purple counterstain.
Western Blot of Rabbit anti-EGFR pY1197 antibody.  
Lane 1: unstimulated A431 cells.  
Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min).  
Load:  30 µg per lane.
Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  134.3 kDa, ~170 kDa for EGFR pY1197. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody, Epidermal growth factor receptor antibody, erbb 1 antibody

EGFR phospho Y1197 Antibody Properties

Description
Anti-EGFR pY1197 (RABBIT) Antibody - 600-401-928
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:20,000
Western Blot : 1:250 - 1:1,500
Immunochemistry: 5 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.55 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
EGFR
Download CofA
Lot#  

EGFR phospho Y1197 Antibody Description

Background
EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development.  Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors.  For example, the epidermal growth factor receptor (EGFR) is mutated and/or overexpressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis.  EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2, ErbB-3/HER-3 and ErbB-4/HER-4. EGFR can heterodimerize with each of the members of this family.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1189-1199 of human EGFR protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kDa in size corresponding to phosphorylated EGFR protein by western blotting in the appropriate cell lysate or extract.   Less than 5.0% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pY1197 of EGFR protein.
Purity/Specifity
This affinity-purified antibody is directed against the phosphorylated form of human EGFR protein at the pY1197 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human EGFR pY1197 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated human EGFR is minimal by ELISA.  A BLAST analysis was used to suggest cross reactivity with phosphorylated EGFR from human, mouse and rat sources.   Reactivity of this antibody with EGFR from other species is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Sartor, C.I. et al., (1997) The Role of EGF Receptor and STAT-3 Activation in Autonomous Proliferation of SUM-102PT Human Breast Cancer Cells. Cancer Research 57:978. Scambia G, et al., (1993) Expression of ras oncogene p21 protein in normal and neoplastic ovarian tissues: Correlation with histopathological features and receptors for estrogen, progesterone, and the epidermal growth factor. Am J Obstet gynecol 168:71-78. Henzen-Logmans, S.C., et al., (1993) Occurrence of epidermal growth factor receeptors in benign and malignant ovarian tumors and normal ovarian tissues: an immunohistochemical study. J Can Res Clin Oncol 188:303-307.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-149

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-905

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry