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Primary Antibodies  >  DNA Damage and Repair Antibodies
 

Mre11 Antibody

Rabbit Polyclonal
Code

600-401-872
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Chromatin Immunoprecipitation (ChIP) using Rockland's Affinity Purified Mre11 (S. cerevisiae) antibody.   A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes).  After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of  sheared chromatin.  Chromatin was immunoprecipitated with the antibody at the stated dilutions.  Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies.  The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus).   PCR products were displayed on a polyacrylamide gel, stained with SyBR  Green® (Invitrogen), and detected using a Fuji scanning fluorometer.  Personal Communication.  Michael Lichten, NIH, CCR, Bethesda, MD.
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Synonyms: AT like disease antibody, Ataxia telangiectasia disorder like antibody, Ataxia-telangiectasia disorder-like antibody, ATLD antibody, DNA recombination and repair protein antibody

Mre11 Antibody Properties

Description
Anti-Mre11 (S. cerevisiae) (RABBIT) Antibody - 600-401-872
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Saccharomyces cerevisiae
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500- 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.19 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MRE11
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Lot#  

Mre11 Antibody Description

Background
Mre11 (also known as double-strand break repair protein MRE11) is a subunit of a complex with Rad50 and Xrs2 (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability.  Mre11 possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity that appears to be required for RMX function.  This nuclear protein is widely conserved and is also involved in meiotic double strand break processing.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 578-590 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and by ChIP assay.  Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that little cross reactivity should be expected with this protein from other sources.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Johzuka K., Ogawa H.; (1995) Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae. Genetics 139:1521-1532. Ogawa H., Johzuka K., Nakagawa T., Leem S.H., Hagihara A.H. (1995) Functions of the yeast meiotic recombination genes, MRE11 and MRE2. Adv. Biophys. 31:67-76. Paull T.T., Gellert M.; (1998) The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks. Mol. Cell 1:969-979.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-458

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-679

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
600-401-896

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry