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Primary Antibodies  >  DNA Damage and Repair Antibodies
 

UBC12 Antibody

Rabbit Polyclonal
Code

600-401-865
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
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Western blot analysis is shown using Rockland's Affinity Purified anti-UBC12 antibody to detect Human UBC12 in various preparations.  This western blot shows reactivity with purified human UBC12 protein (lane 1), NIH-3T3 cells over expressing UBC12 by infection (lane 2) and endogenous UBC12 in NIH 3T3 cells (lane 3).  Peptide competition (lane 4) blocks specific reactivity of the antibody with purified UBC12 protein.  Comparison to a molecular weight marker (not shown) indicates a single band of ~21.0 kDa corresponding to the expected molecular weight for the protein. The blot was blocked with 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 at 4° C overnight.  After washes the blot was incubated with a 1:1,000 dilution of the antibody at room temperature for 2 h in TBS-Tween.  Washes consisted of 3 changes of TBS-Tween buffer for 15 min each.  Detection occurred using HRP anti-Rabbit IgG diluted 1:2,000 and signal processing by chemiluminescence reagent with a 10-sec exposure time.  Other detection systems will yield similar results. Personal communication Martine Roussel.
Western blot analysis is shown using Rockland's Affinity Purified anti-UBC12 antibody to detect Human UBC12 in various preparations.  This western blot shows reactivity with purified human UBC12 protein (lane 1), NIH-3T3 cells over expressing UBC12 by infection (lane 2) and endogenous UBC12 in NIH 3T3 cells (lane 3).  Peptide competition (lane 4) blocks specific reactivity of the antibody with purified UBC12 protein.  Comparison to a molecular weight marker (not shown) indicates a single band of ~21.0 kDa corresponding to the expected molecular weight for the protein. The blot was blocked with 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 at 4° C overnight.  After washes the blot was incubated with a 1:1,000 dilution of the antibody at room temperature for 2 h in TBS-Tween.  Washes consisted of 3 changes of TBS-Tween buffer for 15 min each.  Detection occurred using HRP anti-Rabbit IgG diluted 1:2,000 and signal processing by chemiluminescence reagent with a 10-sec exposure time.  Other detection systems will yield similar results. Personal communication Martine Roussel.
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Synonyms: NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody

UBC12 Antibody Properties

Description
Anti-Human UBC12 (RABBIT) Antibody - 600-401-865
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, chimpanzee, frog, mouse, rat, dog
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.73 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
UBE2M
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UBC12 Antibody Description

Background
UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin-conjugating enzyme family.  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 169-180 of Human UBC12 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
UBC12 Antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
UBC12 Antibody is produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human UBC12 protein. However, 100% homology is on record for this protein from human, chimpanzee, frog, mouse, rat and dog as indicated by BLAST analysis.  Reactivity with UBC12 proteins from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Huang et al., (2004) A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. Nat Struct Mol Biol. Oct;11(10):927-35. Epub 2004 Sep 07. PMID: 1536185 Gong,L. and Yeh,E.T. (1999) Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J. Biol. Chem. 274 (17), 12036-12042. Osaka,F., Kawasaki,H., Aida,N., Saeki,M., Chiba,T., Kawashima,S., Tanaka,K. and Kato,S. (1998) A new NEDD8-ligating system for cullin-4A. Genes Dev. 12 (15), 2263-2268.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-101-342

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-905

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, horse, macaque
Catalog Number
600-401-B05

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry