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Primary Antibodies > Transcription Factor Antibodies

EGR-1 Antibody

Rabbit Polyclonal

Code

600-401-693

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Rockland's Affinity Purified anti-EGR-1 antibody was used at a 10 µg/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.

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Synonyms: AT225 antibody, Early growth response 1 antibody, G0S30 antibody, Krox 24 protein antibody, KROX24 antibody, Nerve growth factor-induced protein A antibody

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EGR-1 Antibody Properties

Description

Anti-EGR-1 (RABBIT) Antibody - 600-401-693

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, chimpanzee, mouse

Clonality

Polyclonal

Application

ELISA : 1:4,000 - 1:16,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 2 µg/ml to 20 µg/ml
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

0.93 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

EGR-1

EGR-1 Antibody Description

Background

EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation. EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 (eqpyehltaesfpdi) of Human EGR-1.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity purified antibody is directed against human EGR-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Suggs,S.V., Katzowitz,J.L., Tsai-Morris,C. and Sukhatme,V.P. (1990) cDNA sequence of the human cellular early growth response gene Egr-1. Nucleic Acids Res. 18 (14), 4283. Shimizu,N., Ohta,M., Fujiwara,C., Sagara,J., Mochizuki,N., Oda,T. and Utiyama,H. (1992) A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation. J. Biochem. 111 (2), 272-277. Wright,J.J., Gunter,K.C., Mitsuya,H., Irving,S.G., Kelly,K. and Siebenlist,U. (1990) Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells. Science 248 (4955), 588-591.

Specific Reference

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Antigen Retrieval Methods

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