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Primary Antibodies  >  Phospho Specific Antibodies
 

Aurora B Phospho pT232 Antibody

Rabbit Polyclonal
Code

600-401-677
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size).  All lanes : Aurora B (phospho T232) antibody diluted 1:500.  Lane 1 :  Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs).  Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) and with the phosphopeptide immunogen.
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Synonyms: Phospho Aurora B, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

Aurora B Phospho pT232 Antibody Properties

Description
Anti-Aurora B pT232 (RABBIT) Antibody - 600-401-677
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
IF Microscopy : User Optimized
Western Blot : 1:250 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
AURKB
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Lot#  

Aurora B Phospho pT232 Antibody Description

Background
Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body.  High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store Phospho Specific Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Phospho pT232 Aurora B antibody has been tested for use in ELISA and by western blot.  See below for specific protocol.  Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract.  HeLa cell lysate can be used as a positive control.
Purity/Specifity
Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein.  Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA.  No reaction is expected against Aurora Kinase A.  However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee.  Cross reactivity with Aurora Kinase B from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wang, F., Ulyanova, N. P., Daum, J. R., Patnaik, D., Kateneva, A. V., Gorbsky, G. J., & Higgins, J. M. (2012). Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation. The Journal of cell biology, 199(2), 251-268.
Specific Reference
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Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
200-301-880

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Human
Catalog Number
200-C01-B33

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-465

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry