Anti-Cyclin B1 pS126 Antibody - Western Blot

Datasheet

Cyclin B1 phospho S126 Antibody

Name: Cyclin B1 phospho S126 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 600-401-464

Size: 100 µg

Applications: WB, ELISA

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

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Background

Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

Expand Background

Specifications for Cyclin B1 phospho S126 Antibody

Product Details

Description: Anti-Cyclin B1 pS126 (RABBIT) Antibody - 600-401-464

Synonyms: rabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: CCNB1 - View All CCNB1 Products

Reactivity: Human

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.

Purity/Specificity: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.

Database Links:

UniProtKB - P14635

GeneID - 891

Application Details

Tested Applications: ELISA, WB

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 2.01 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.