Datasheet

MAD2L1 Antibody

Name: MAD2L1 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 600-401-461

Size: 100 µg

Applications: WB, ELISA

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

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Background

MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. This protein has a nuclear localization.

Expand Background

Specifications for MAD2L1 Antibody

Product Details

Description: Anti-MAD2L1 (RABBIT) Antibody - 600-401-461

Synonyms: rabbit anti-MAD2L1 antibody, MAD2, MAD2-like protein 1, HsMAD2, Mitotic arrest deficient 2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: MAD2L1 - View All MAD2L1 Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal region near amino acid residues 1-25 of Human MAD2L1 protein.

Purity/Specificity: This affinity purified antibody is directed against human MAD2L1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, dog, macaque, chimpanzee and gecko based on 100% homology for the immunogen sequence. Cross reactivity with MAD2L1 may occur from mouse and chicken sources, as only a two amino acid residue change is found within the immunogen sequence (90% positive by BLAST). Cross reactivity with MAD2L1 homologues from other sources has not been determined.

Database Links:

UniProtKB - Q13257

NCBI - 4505067

GeneID - 4085

Application Details

Tested Applications: ELISA, WB

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.19 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.