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Primary Antibodies > Kinase and Phosphatase Antibodies

GSK3 Alpha Antibody

Rabbit Polyclonal

Code

600-401-444

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A present in ~ 35 µg of HEK293 whole cell lysate (lane 1). A 4-20% Tris Glycine gel was used for separation followed by blocking with 5% BLOTTO in PBS. Primary antibody was used at a 1:1,000 dilution in blocking buffer and reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

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Synonyms: GSK 3 alpha antibody, DKFZp686D0638 antibody, Glycogen synthase kinase 3 alpha antibody, GSK 3 alpha antibody, GSK 3A antibody, GSK3A antibody, GSK3alpha antibody

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GSK3 Alpha Antibody Properties

Description

Anti-GSK3 alpha (RABBIT) Antibody - 600-401-444

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:4,000 - 1:20,000
IF Microscopy : User Optimized
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

GSK3A

GSK3 Alpha Antibody Description

Background

Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily; gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun. GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 16-25 of human GSK3 alpha.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity-purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity-purified antibody is directed against human GSK3A. The product was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody will react with both the phosphorylated and non-phosphorylated form of the protein at the S21residue. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100% homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined. No cross reactivity is expected against GSK3B.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Gaster,M., et al. (2004) The primary defect in glycogen synthase activity is not based on increased glycogen synthase kinase-3alpha activity in diabetic myotubes. Biochem. Biophys. Res. Commun. 319 (4), 1235-1240. Barry,F.A., Graham,G.J., Fry,M.J. and Gibbins,J.M. (2003) Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?. FEBS Lett. 553 (1-2), 173-178. Nikoulina,S.E., Ciaraldi,T.P., Mudaliar,S., Carter,L., Johnson,K. and Henry,R.R. (2002) Inhibition of glycogen synthase kinase 3 improves insulin action and glucose metabolism in human skeletal muscle. Diabetes 51 (7), 2190-2198. He,X., Saint-Jeannet,J.P., Woodgett,J.R., Varmus,H.E. and Dawid,I.B. (1995) Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos. Nature 374 (6523), 617-622.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra