Datasheet

GSK3 Alpha phospho S21 Antibody

Name: GSK3 Alpha phospho S21 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 600-401-429

Size: 100 µg

Applications: WB, ELISA

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

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Background

Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily; gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun. GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

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Specifications for GSK3 Alpha phospho S21 Antibody

Product Details

Description: Anti-GSK3 alpha pS21 (RABBIT) Antibody - 600-401-429

Synonyms: rabbit anti-GSK3 alpha pS21 antibody, rabbit anti-GSK3α pS21 antibody, GSK 3 alpha pS21 antibody, Glycogen synthase kinase-3 alpha antibody, phosphorylated GSK-3 alpha antibody, GSK 3A antibody, GSK-3A antibody, GSK3alpha antibody, Serine/threonine-protein kinase GSK3A

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: GSK3A - View All GSK3A Products

Reactivity: Human

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.

Purity/Specificity: This affinity-purified antibody is directed against the phosphorylated form of human GSK3A at the pS 21 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100% homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined.

Database Links:

NCBI - 49574532

UniProtKB - P49840

GeneID - 2931

Application Details

Tested Applications: ELISA, WB

Application Note: This affinity-purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract. Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS21 of GSK3A.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.28 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.