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Primary Antibodies  >  Phospho Specific Antibodies
 

GSK3 Beta phospho S9 Antibody

Rabbit Polyclonal
Code

600-401-424
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Rabbit anti-GSK3B pS9 was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded.  Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
Western blot using ROCKLAND Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution.  All lanes contain human 293T whole cell lysate showing a band at 47 kDa.  Cells were serum starved for 24 h prior to extraction.  Key:  Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20' , lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'.   Molecular weight markers confirm a MW of ~ 49 kDa.  Use a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (611-103-122) for detection.
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Synonyms: Glycogen Synthase Kinase 3 Beta antibody, GSK 3 beta antibody, GSK 3B antibody, GSK3B antibody, GSK3B protein antibody, GSK3beta isoform antibody

GSK3 Beta phospho S9 Antibody Properties

Description
Anti-GSK3 beta pS9 (RABBIT) Antibody - 600-401-424
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
IF Microscopy : 1:200 - 1:1,000
Western Blot : 1:500 - 1:3,000
Immunochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GSK3B
Download CofA
Lot#  

GSK3 Beta phospho S9 Antibody Description

Background
Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.  GSK 3B participates in the Wnt signaling pathway and has been implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.  GSK 3B phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the aa 4-12 of human GSK3 beta.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.  Serum starved 293T whole cell lysate is suitable for use as a positive control.  Anti-GSK 3B shows a strong signal to GSK 3B in westerns at the estimated molecular weight of 47 kD.  This antibody may show very weak reactivity against GSK 3A.
Purity/Specifity
This affinity purified antibody is directed against the phosphorylated form of human GSK3B at the pS9 residue.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.  This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal.  A BLAST analysis was used to suggest reactivity with this protein from human, rat, frog, chicken, dog and zebrafish based on 100% homology for the immunogen sequence. Cross reactivity with GSK3B homologues from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Stambolic V. and Woodgett J.R. (1994) Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Biochem. J. 303:701-704. Rhoads A.R. et al. (1999) Radiation hybrid mapping of genes in the lithium-sensitive wnt signaling pathway. Mol. Psychiatry 4:437-442. Lau K.F. et al. (1999) Molecular cloning and characterization of the human glycogen synthase kinase-3beta promoter. Genomics 60:121-128.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, ChIP
Reacts With
human
Catalog Number
200-301-174

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry