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Primary Antibodies  >  Kinase and Phosphatase Antibodies
 

MAPKAP Kinase 2 Antibody

Rabbit Polyclonal
Code

600-401-281
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Affinity purified anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by western blot.  Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution to detect 20 ug of whole cell lysate containing MK2. This antibody detects a single 44 kDa protein as indicated in crude extracts prepared from either unstimulated or TNFa stimulated human HeLa cell lysates.   A 4-20% gradient gel was used to separate the protein by SDS-PAGE.  The protein was transferred to nitrocellulose using standard methods.  After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature.  LICOR's Odyssey®  Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
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Synonyms: MAPKAP kinase-2, MK2, mitogen-activated protein kinase-activated protein kinase-2

MAPKAP Kinase 2 Antibody Properties

Description
Anti-MAPKAP Kinase 2 (RABBIT) Antibody - 600-401-281
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:15,000
Western Blot : 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.9 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MAPKAPK2
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Lot#  

MAPKAP Kinase 2 Antibody Description

Background
MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay.  MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites.  MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The best-characterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance.  MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNFa at a post-transcriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.
Immunogen
Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to aa 310-325 of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody is suitable for western blotting, ELISA, immunoprecipitation and kinase assays.  Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. The antibody detects a single 44 kDa protein in crude extracts prepared either from unstimulated (p/n w09-000-364) or TNFa stimulated ((p/n w09-000-367) human HeLa cell lysates. A 1:15,000 dilution is recommended for ELISA. The researcher should determine optimal titers for other applications.
Purity/Specifity
This affinity-purified antibody is directed against rabbit MAPKAP Kinase 2 and is useful in determining its presence in various assays. This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts.  This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish.  Cross reactivity with MAPKAP Kinase 2 from other sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Kotlyarov A, Neininger A, Schubert C, Eckert R, Birchmeier C, Volk HD, Gaestel M. (1999) MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis. Nat Cell Biol. Jun;1(2):94-7. Cheng TJ , Tseng YF , Chang WM , Chang MD , Lai YK, (2003) Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2. J Cell Biochem, 89(3): 589-602. Rousseau S , Morrice N , Peggie M , Campbell DG , Gaestel M , Cohen P, (2002) Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs. EMBO J, 21(23): 6505-14.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-149

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-1102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-3102

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry