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Primary Antibodies > Phospho Specific Antibodies

AKT phospho T308 Antibody

Rabbit Polyclonal

Code

600-401-269

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western Blot of Rabbit anti-Akt phospho T308 antibody.
Lane 1: GST tagged AKT1 un-active recombinant protein.
Lane 2: GST tagged AKT1 active recombinant protein.
Load: 50 ng per lane.
Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C.
Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT.
Block: MB-070 for 30 min at RT.
Other band(s): none.

Shipping & Handling Fees Apply.

Shipping:

$85.00 to United States

Handling:

$45.00

Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

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AKT phospho T308 Antibody Properties

Description

Anti-AKT pT308 (RABBIT) Antibody - 600-401-269

Host

Rabbit

Target Species

Human

Known Cross Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Application

ELISA : 1:15,000
Western Blot : User Optimized
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

.44 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

AKT1

AKT phospho T308 Antibody Description

Background

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Immunogen

Anti-AKT phospho T308 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to aa 302-311 of human AKT protein conjugated to KLH.

Immunogen Type

Peptide

Post Translational Modification

Phosphorylation

Storage Condition

Store AKT phospho T308 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

AKT antibody is phospho specific for pT308 and is suitable for western blotting and ELISA.

Purity/Specifity

AKT phospho T308 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Hedjazifar,S., Jenndahl,L.E., Shimokawa,H. and Baeckstrom,D. (2005) PKB mediates c-erbB2-induced epithelial beta1 integrin conformational inactivation through Rho-independent F-actin rearrangements. Exp. Cell Res. 307 (1), 259-275. Saji,M., Vasko,V., Kada,F., Allbritton,E.H., Burman,K.D. and Ringel,M.D. (2005) Akt1 contains a functional leucine-rich nuclear export sequence. Biochem. Biophys. Res. Commun. 332 (1), 167-173. Sutherland,B.W., Kucab,J., Wu,J., Lee,C., Cheang,M.C., Yorida,E., Turbin,D., Dedhar,S., Nelson,C., Pollak,M., Leighton Grimes,H., Miller,K., Badve,S., Huntsman,D., Blake-Gilks,C., Chen,M., Pallen,C.J. and Dunn,S.E. (2005) Akt phosphorylates the Y-box binding protein 1 at Ser102 located in the cold shock domain and affects the anchorage-independent growth of breast cancer cells. Oncogene 24 (26), 4281-4292.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra