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COLLAGEN Type III Antibody

Rabbit Polyclonal
NCI Collaboration
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Rockland anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.  The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining.   Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
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Number:
600-401-105-0.5
Size:
500 µg
Price:
$609.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

COLLAGEN Type III Antibody Properties

Description
Anti-Collagen Type III (RABBIT) Antibody - 600-401-105-0.5
Host
Rabbit
Target Species
Mammalian
Known Cross Reactivity
human, bovine
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:50,000
Western Blot : 1:1,000 - 1:10,000
Immunohistochemistry: 1:50 - 1:200
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Reconstitution Volume
500 µL
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
COL3A1
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COLLAGEN Type III Antibody Description

Background
Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Synonyms
Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody
Immunogen
Collagen Type III from human and bovine placenta
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening.  This product is stable at 4° C as a lyophilized powder.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Application Note
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
Purity/Specifity
Anti-Collagen Type III has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Mak KM, Kwong AJ, Chu E, Hoo NM. (2011) Hepatic Steatosis, Fibrosis, and Cancer in Elderly Cadavers. Cancer Biology. 5 DEC 2011. DOI: 10.1002/ar.21525.
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
001-001-105

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
009-001-105

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, bovine
Catalog Number
600-401-105-0.1

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, bovine
Catalog Number
600-406-105

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry