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Primary Antibodies  >  Serum Protein Component Antibodies
 

Human Serum Albumin Antibody

Rabbit Polyclonal
Code

600-401-033
Size

1 mg
Price

$309.00
Availability

Ships next business day.
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Western Blot of human serum albumin.  
Lane 1:  1:400 of FBS.  
Lane 2:  1:1600 of FBS. 
Lane 3:  1:400 of normal rabbit serum.  
Lane 4:  1:1600 of normal rabbit serum. 
Lane 5:  1:400 of normal human serum.  
Lane 6:  1:1600 of normal human serum.   
Lane 7:  1:10 of human serum albumin (10mg/mL).  
Lane 8:  1:40 of human serum albumin (10mg/mL).  
Lane 9:  molecular weight.  
Right Blot: Secondary only incubated for control.  
Primary antibody:  human serum albumin at 1:400 for overnight at 4°C.
Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT.  Dylight 649 conjugated Stretavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent.
Block:  1.5 % rabbit serum overnight at 4°C.
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Shipping:
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Handling:
$20.00
Synonyms: Albumin antibody, Analbuminemia antibody, Bisalbuminemia antibody, Cell growth inhibiting protein 42 antibody, DKFZp779N1935 antibody, Dysalbuminemic hyperthyroxinemia antibody, Growth inhibiting protein 20 antibody, HSA antibody

Human Serum Albumin Antibody Properties

Description
Anti-HUMAN SERUM ALBUMIN (RABBIT) Antibody - 600-401-033
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

Human Serum Albumin Antibody Description

Background
Human Serum Albumin Antibody detects serum albumin which is the most abundant protein in human blood plasma. Serum albumin is produced in the liver and constitutes about half of the blood serum protein. It is soluble and monomeric, and is shown to transport hormones, fatty acids, and other compounds, buffers pH, and maintains osmotic pressure, among other functions. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. The reference range for albumin concentrations in blood is 3.4 to 5.4 g/dL. Serum Albimin has a molecular mass of 67 kDa. This antibody is suitable for researchers in cardiovascular research, Serum Protein Component research, as well as general research.
Immunogen
Human Serum Albumin
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Human Serum Albumin Antibody is suitable for the detection of human serum albumin in blood and tissue samples. Antibody is suitable for western blotting to detect a single band of 67 kDa, the expected apparent molecular weight, and for use in ELISA. Researchers should determine optimal titers for applications that are not stated below.
Purity/Specifity
Anti-Human Serum Albumin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.  Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
FLISA, WB, IF
Reacts With
human
Catalog Number
600-402-033

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-403-033

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-406-033

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry