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Primary Antibodies  >  Infectious Disease Antibodies
 

Interferon Beta Antibody

Rabbit Polyclonal
Code

214-401-C40
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's protein-A purified anti-swine IFN beta antibody shows detection of recombinant  Swine IFN beta at about 19.5 kDa raised in yeast.  Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C.   After washing, membrane was probed with IRDye800® Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) for 45 min at room temperature.
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Synonyms: Interferon-beta, interferon-beta/ poIFN-beta, IFN

Interferon Beta Antibody Properties

Description
Anti-Swine Interferon Beta (RABBIT) Antibody - 214-401-C40
Host
Rabbit
Target Species
Swine
Known Cross Reactivity
Swine
Clonality
Polyclonal
Application
ELISA : 1:20,000
Western Blot : 1:500-1:2,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IFN beta
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Interferon Beta Antibody Description

Background
Type I interferons comprise a vast and growing group of IFN proteins. Homologous molecules to type I IFNs are found in many species, including all mammals, and some have been identified in birds, reptiles, amphibians and fish species. The mammalian types are designated IFN-alpha, IFN-beta, IFN-kappa, IFN-delta, IFN-epsilon, IFN-tau, IFN-omega, and IFN-zeta (also known as limitin). The IFN-beta proteins are produced in large quantities by fibroblasts. They have antiviral activity which is mainly involved in innate immune response. Two types of IFN-beta proteins have been described.
Immunogen
This protein A purified antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 164 amino acids of the mature swine IFN beta protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.5 kDa in size corresponding to swine IFN beta by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was protein A purified from monospecific antiserum by chomotography. This antibody is specific for swine IFN beta protein. A BLAST analysis was used to suggest cross-reactivity with IFN beta from Sus scrofa (pig), Potamochoerus larvatus koiropotamus, Potamochoerus larvatus (Bushpig), and Phacochoerus africanus (warthog) sources based on 100% homology with the immunizing sequence. Cross-reactivity with IFN beta from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
de Veer MJ., Holko M., Frevel M., Walker E., Der S., Paranjape JM., Silverman RH., Williams BR. (2001). Functional classification of interferon-stimulated genes identified using microarrays. Journal of leukocyte biology 69 (6): 912-20. Goldstein D.; Laszlo J. (Sep 1988). The role of interferon in cancer therapy: a current perspective. CA: a cancer journal for clinicians 38 (5): 258-77.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-310

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-310

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bovine
Catalog Number
201-401-C41

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-C64

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry