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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-17A Antibody

Rabbit Polyclonal
Code

212-401-B32
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's anti-IL-17A antibody shows detection of mouse recombinant IL-17A protein (arrowhead, lane 1).  Approximately 2 µg of recombinant protein was loaded onto the gel.  Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG (p/n 611-143-122).  Molecular weight estimation was made by comparison to prestained MW markers indicated at the left.  Other detection systems will yield similar results.
Western blot using Rockland's anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1).  Approximately 2 µg of recombinant protein was loaded onto the gel.  Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG (p/n 611-143-122).  Molecular weight estimation was made by comparison to prestained MW markers indicated at the left.  Other detection systems will yield similar results.
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Synonyms: Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8

IL-17A Antibody Properties

Description
Anti-Rat IL-17A (RABBIT) Antibody - 212-401-B32
Host
Rabbit
Target Species
Rat
Known Cross Reactivity
rat and mouse
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
IL17A
Download CofA
Lot#  

IL-17A Antibody Description

Background
Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. The recombinant immunogen is a non-glycosylated homodimer joined by disulfide bonds having a molecular mass of 30.0 kDa. By western blot from a reducing gel expect a band approximately 15.0 kDa in size corresponding to rat IL17-A protein in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Rouvier,E., Luciani,M.F., Mattei,M.G., Denizot,F. and Golstein,P. (1993) CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene. J. Immunol. 150 (12), 5445-5456. Yao,Z., Timour,M., Painter,S., Fanslow,W. and Spriggs,M. (1996) Complete nucleotide sequence of the mouse CTLA8 gene. Gene 168 (2), 223-225. Kennedy,J., Rossi,D.L., Zurawski,S.M., Vega,F. Jr., Kastelein,R.A., Wagner,J.L., Hannum,C.H. and Zlotnik,A. (1996) Mouse IL-17: a cytokine preferentially expressed by alpha beta TCR + CD4-CD8-T cells. J. Interferon Cytokine Res. 16 (8), 611-617.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Rat
Catalog Number
012-001-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
human
Catalog Number
209-301-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B32

Product Type
Primary Antibodies;
Applications
ELISA
Reacts With
rat and mouse
Catalog Number
212-403-B32

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry