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IL-17F Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's anti-Mouse IL-17F antibody shows detection of a band ~18 kDa in size corresponding to recombinant mouse IL-17F.  Recombinant mouse IL-17F was loaded on to an SDS-PAGE gel at 0.25 µg and after separation was transferred to nitrocellulose.  The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C.  After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate (p/n FEMTOMAX-110).  Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
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Number:
210-401-B31
Size:
100 µg
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

IL-17F Antibody Properties

Description
Anti-Mouse IL-17F (RABBIT) Antibody - 210-401-B31
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
Il17f
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IL-17F Antibody Description

Background
IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. Anti-IL-17F antibody is ideal for investigators involved in Cardiovascular and Immunology research.
Synonyms
IL-17F, Interleukin-17F, ML1, ML-1, IL17F, and IL24
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store anti-IL-17F antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. IL-17F forms a disulfide linked homodimer. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
IL-17F antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Gomez-Rodriguez,J., Sahu,N., Handon,R., Davidson,T.S., Anderson,S.M., Kirby,M.R., August,A. and Schwartzberg,P.L. (2009) Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase. Immunity 31 (4), 587-597. Nowak,E.C., Weaver,C.T., Turner,H., Begum-Haque,S., Becher,B., Schreiner,B., Coyle,A.J., Kasper,L.H. and Noelle,R.J. (2009) IL-9 as a mediator of Th17-driven inflammatory disease. J. Exp. Med. 206 (8), 1653-1660. Yu,Y., Iclozan,C., Yamazaki,T., Yang,X., Anasetti,C., Dong,C. and Yu,X.Z. (2009) Abundant c-Fas-associated death domain-like interleukin-1-converting enzyme inhibitory protein expression determines resistance of T helper 17 cells to activation-induced cell death. Blood 114 (5), 1026-1028. (2009) Hymowitz,S.G., Filvaroff,E.H., Yin,J.P., Lee,J., Cai,L., Risser,P., Maruoka,M., Mao,W., Foster,J., Kelley,R.F., Pan,G., Gurney,A.L., de Vos,A.M. and Starovasnik,M.A. (2001) IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding. EMBO J. 20 (19), 5332-5341.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-B31

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-301-B31

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B31

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, rat
Catalog Number
210-403-B31

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry