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Secondary Antibodies  >  IgG Fraction Antibodies
 

Human IgM mu chain Antibody Peroxidase Conjugated

Rabbit Polyclonal
NCI Collaboration
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Number:
209-4307
Size:
20 mg
Price:
$195.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Human IgM mu chain Antibody Peroxidase Conjugated Properties

Description
Anti-HUMAN IgM mu chain (RABBIT) Antibody Peroxidase Conjugated - 209-4307
Host
Rabbit
Specificity
IgM µ chain
Target Species
Human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:1,000 - 1:10,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Concentration
10.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Human IgM mu chain Antibody Peroxidase Conjugated Description

Synonyms
HRP
Immunogen
Human IgM mu heavy chain
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
This product has been assayed against 1.0 ug of Human IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. 
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgM and Human Serum.  No reaction was observed against Human IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate