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Primary Antibodies > Cytokines & Growth Factor Antibodies

IL-3 Antibody Peroxidase Conjugated

Rabbit Polyclonal

Code

209-403-B92

Size

100 µg

Price

$294.00

Availability

Ships within 2 weeks.

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Synonyms: Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL 3, Interleukin 3

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IL-3 Antibody Peroxidase Conjugated Properties

Description

Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated - 209-403-B92

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:10,000 - 1:50,000
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:2,500

Physical State

Lyophilized

Shipping condition

Ambient

Format

IgG

Label

Peroxidase (Horseradish)

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

100 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Stabilizer

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

GeneName

IL3

IL-3 Antibody Peroxidase Conjugated Description

Background

The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.

Immunogen

Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This IL-3 antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Purity/Specifity

Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Davila,S., Froeling,F.E., Tan,A., Bonnard,C., Boland,G.J., Snippe,H., Hibberd,M.L. and Seielstad,M. (2010) New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun. 11 (3), 232-238. Simmonds,M.J., Yesmin,K., Newby,P.R., Brand,O.J., Franklyn,J.A. and Gough,S.C. (2010) Confirmation of association of chromosome 5q31-33 with United Kingdom Caucasian Graves' disease. Thyroid 20 (4), 413-417. Zhu,W., Liu,N., Zhao,Y., Jia,H., Cui,B. and Ning,G. (2010) Association analysis of polymorphisms in IL-3, IL-4, IL-5, IL-9 and IL-13 with Graves' disease. J. Endocrinol. Invest. (2010) In press

Specific Reference

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