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VEGF-121 Antibody

Rabbit Polyclonal
NCI Collaboration
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Anti-human VEGF-121 by western blot shows detection of recombinant Human VEGF-121 raised in E.coli.  Recombinant (0.1  µg, 28.4 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to  nitrocellulose.  The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's, Inc. Anti-Human VEGF-121.   After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT.  Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
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Number:
209-401-C23
Size:
100 µg
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

VEGF-121 Antibody Properties

Description
Anti-Human VEGF-121 (RABBIT) Antibody - 209-401-C23
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
VEGFA
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VEGF-121 Antibody Description

Background
Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth, vascular permeability, cell migration, and endothelial cell proliferation and growth, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways and in inhibition of apoptosis. VEGF binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF121 is acidic, freely secreted, and widely expressed. This isoform is produced by alternative promoter usage and alternative initiation. It starts at an alternative upstream CUG codon and is post-translationally processed to produce the secreted VEGF peptide and a N-terminal peptide N-VEGF. The unprocessed protein and the N-VEGF peptide may localize to the nucleus, the endoplasmic reticulum and the Golgi or the extracellular matrix.
Synonyms
Vascular endothelial growth factor A-121, VEGF-A121 cytokine, Vascular permeability factor, VPF-121, VEGFA-121
Immunogen
This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human VEGFA-121 protein produced in E.coli.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14.7 Da in size corresponding to the mature human VEGFA-121 protein by western blotting in appropriate cell lysate or extract.
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human VEGFA-121 protein. A BLAST analysis was used to suggest cross-reactivity with VEGFA-121 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to VEGFA-121 from a wide variety of animals. Cross-reactivity with VEGFA-121 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Sato,J.D. and Whitney,R.G. (1999) Direct Submission: Human cDNA for vascular endothelial growth factor isoform VEGF121. Unpublished Submitted: Division of Cell, Molecular and Developmental Biology, American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110, USA Rosenbaum-Dekel Y., Fuchs A., Yakirevich E., Azriel A., Mazareb S., Resnick M.B., Levi B.Z. (2005) Nuclear localization of long-VEGF is associated with hypoxia and tumor angiogenesis. Biochem. Biophys. Res. Commun. 332:271-278 [PubMed: 15896327] [Abstract]
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-C23

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bovine
Catalog Number
200-401-B74

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B99

Product Type
Primary Antibodies;
Applications
WB, IHC
Reacts With
human
Catalog Number
209-403-B99

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry