House Buffer
 
You may also want to consider these related products.
Anti-Human IL-17F (RABBIT) Antibody - 209-401-B31
Anti-Human IL-17F (RABBIT) Antibody - 209-401-B31
Anti-Human IL-17A (RABBIT) Antibody - 209-401-B32
Anti-Human IL-17A (RABBIT) Antibody - 209-401-B32
Anti-Human IL-17A (RABBIT) Antibody Peroxidase Conjugated - 209-403-B32
Anti-Human IL-17A (RABBIT) Antibody Peroxidase Conjugated - 209-403-B32
Anti-Rat IL-17A (RABBIT) Antibody - 212-401-B32
Anti-Rat IL-17A (RABBIT) Antibody - 212-401-B32
Primary Antibodies  >  Sample Size Primary Antibodies
 

IL-17A Antibody

Rabbit Polyclonal
Code

209-401-B32S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western blot using Rockland's anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M).  After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.  Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070).  Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Interleukin-17, and in rodents only: Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8

IL-17A Antibody Properties

Description
Anti-Human IL-17A (RABBIT) Antibody - 209-401-B32S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:1:000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL17A
Download CofA
Lot#  

IL-17A Antibody Description

Background
IL17-A (also known as Interleukin-17) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL-17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. Anti-IL-17 antibody is ideal for investigators involved in cytokines, growth factors, cancer, and immunology reseach.
Immunogen
IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
IL-17-A antibody has been tested for use in ELISA and western blotting. By western blot a band approximately ~17.5 kDa in size corresponding to human IL-17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-IL-17A Antibody was affinity purified from monospecific antiserum by Protein A Purification. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Shin,J.H., Shin,D.W. and Noh,M. (2009) Interleukin-17A inhibits adipocyte differentiation in human mesenchymal stem cells and regulates pro-inflammatory responses in adipocytes. Biochem. Pharmacol. 77 (12), 1835-1844. Yin,Y., Wen,S., Li,G. and Wang,D. (2009) Hypoxia enhances stimulating effect of amyloid beta peptide (25-35) for interleukin 17 and T helper lymphocyte subtype 17 upregulation in cultured peripheral blood mononuclear cells. Microbiol. Immunol. 53 (5), 281-286. Paust,H.J., Turner,J.E., Steinmetz,O.M., Peters,A., Heymann,F., Holscher,C., Wolf,G., Kurts,C., Mittrucker,H.W., Stahl,R.A. and Panzer,U. (2009) The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis. J. Am. Soc. Nephrol. 20 (5), 969-979.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B31

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Human
Catalog Number
209-403-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
rat and mouse
Catalog Number
212-401-B32

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry