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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-6 Antibody

Mouse Monoclonal 33A12.G9 IgG1 kappa
Code

209-301-310
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Flow Cytometry of Human anti-IL-6 antibody 209-301-310
Cells: human PBMC
Stimulation: Figure A: un-stimulated, Figure B: 1 µg/mL LPS in a protein transport inhibitor for 5 hours
Staining: (surface) x-axis: anti-CD14, (intracellular) y-axis: anti-IL-6
Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody (p/n 209-301-310) was used at 1:1000 in 1% BSA-TTBS over night at 4°C.  Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP.  Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
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Shipping:
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Handling:
$20.00
Synonyms: Anti-IL 6, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF

IL-6 Antibody Properties

Description
Anti-Human IL-6 (MOUSE) Monoclonal Antibody - 209-301-310
Host
Mouse
Target Species
Human
Known Cross Reactivity
Human
Clonality
Monoclonal 33A12.G9 IgG1 kappa
Application
ELISA : 1:10,000
FlowCytometry : 0.5 mg/mL
Western Blot : 1:1000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL6
Download CofA
Lot#  

IL-6 Antibody Description

Background
IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
Immunogen
This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store IL 6 antibody at 4° C . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-IL-6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Novick, D., et al. (1989) Monoclonal antibodies for affinity Purification of IL-6/IFNveta 2 and for neutralization of HGF activity. Hybridoma 8: 561-567. Novick, D., et al. (1992) Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody. Cytokine 4 (1): 6-11. Silvera, M.R., et al. (1994) Expression of TGFa isoforms by thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGFa as a regulator of IL-dependent stimulation of IL-6. Lymphokine Cytokine Res 13: 277-285
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-301-B31

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
human
Catalog Number
209-301-B32

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
210-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry