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IL-1F5 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast.  The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane.   Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C.   After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) for 45 min at room temperature.
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Number:
201-401-B77
Size:
100 µg
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

IL-1F5 Antibody Properties

Description
Anti-Bovine IL-1F5 (RABBIT) Antibody - 201-401-B77
Host
Rabbit
Target Species
Bovine
Known Cross Reactivity
Bovine
Clonality
Polyclonal
Application
ELISA : 8,000
Western Blot : 1 µg/mL
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
Concentration
1.0 µg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL1F5
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IL-1F5 Antibody Description

Background
Interleukin-1F5 is a recently discovered cytokine which was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6) in humans. There is very little published regarding the bovine IL1F5 protein. In the human, this gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Expression of the gene appears to be restricted to various epithelial cell populations. Costelloe et al (2008) have identified SIGIRR [single immunoglobulin domain-containing IL1R-related protein] as a receptor for human IL1F5. Anti-IL-1F5 antibody antibody is ideal for investigators involved in Signal Transduction and Immunology research.
Synonyms
Interleukin 1 family, member 5 (Delta) . In humans it is also known as: IL1RP3, Il1hy1, Il-1h3, MGC29840, FIL1(DELTA), IL1F5, Il1f5, IL1L1, Fil1delta, IL1HY1, FIL1D, FIL1, or AI413231.
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kDa in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-1F5 protein. A BLAST analysis was used to suggest cross-reactivity with IL-1F5 from dog, panda, and ferret based on 90-92% homology with the immunizing sequence.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Costelloe C et al IL-1F5 mediates anti-inflammatory activity in the brain through induction of IL-4 following interaction with SIGIRR/TIR8. Journal of Neurochemistry 105(5): 1960-1969 (2008).
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
001-001-103

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, Neu, FC
Reacts With
human, primate
Catalog Number
109-401-304

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, primate
Catalog Number
109-401-305

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
bovine, swine, horse
Catalog Number
200-401-B53

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry