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Beta Galactosidase Antibody Fluorescein Conjugated

Rabbit Polyclonal
NCI Collaboration
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Western blotting using Rockland's Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead).   Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate.  Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070).  The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000.  Reaction occurred for 2 hours at room temperature.  Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
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Number:
200-4236
Size:
10 mg
Price:
$1,995.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Beta Galactosidase Antibody Fluorescein Conjugated Properties

Description
Anti-BETA GALACTOSIDASE (E.coli) (RABBIT) Antibody Fluorescein Conjugated - 200-4236
Host
Rabbit
Clonality
Polyclonal
Application
IF Microscopy : 1:500 - 1:2,500
Western Blot : 1:10,000
Other Dilution: User Optimized
Epitope Tag Type
b-GAL
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
FP Value
3.5 moles Fluorescein (FITC) per mole of IgG
Concentration
5.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Beta Galactosidase Antibody Fluorescein Conjugated Description

Background
This product is designed for fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms
FITC
Immunogen
Beta Galactosidase (E. coli)
Immunogen Type
Native Protein
Storage Condition
Store vial antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Suitable for fluorescent western blotting.
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Beta Galactosidase (E. coli).
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate