Beta Galactosidase Antibody
Anti-b-Galactosidase Antibody - Western Blot
Anti-b-Gala Antibody - Western Blot
Western Blot of Anti-b-Gala Antibody
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Beta Galactosidase Antibody

Rabbit Polyclonal

17 References
200-4136 200-4136S 200-4136-0100
10 mg 25 µL 100 µg
WB, ELISA, IHC, IF, Multiplex
b-GAL
Rabbit
Beta Galactosidase Antibody Fluorescein Conjugated Beta Galactosidase Antibody Peroxidase Conjugated Beta Galactosidase Antibody Biotin Conjugated Beta Galactosidase Antibody Fluorescein Conjugated
Please contact us for pricing
$125.00 /Per Item
$445.00 /Per Item
10 mg $0.00
25 µL $125.00
100 µg $445.00
Request a Quote
Bulk Orders Bulk Orders Bulk Orders
Availability: Ships next business day Availability: Ships next business day Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

Product Details

Anti-Beta Galactosidase (E. coli) (RABBIT) Antibody (BULK ORDER) - 200-4136
rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal antibody, ß-Gal, Anti-ß-Gal Antibody
Rabbit
Polyclonal
IgG

Target Details

lacZ  - View All lacZ Products
b-GAL
Native Protein
Full length native Beta Galactosidase isolated from E.coli
Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli].  Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined.  Very low background staining has been reported in various assays.
NCBI - NP_414878.1
UniProtKB - P00722

Application Details

ELISA, WB
IF, IHC, Multiplex  - View References
Anti-Beta-Gal Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis.   A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.

Formulation

Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). A Group of olfactory Receptor Alleles that encode full Length proteins are Down-Regulated as olfactory Sensory neurons Mature. Sci Rep.
Applications
IF, Confocal Microscopy
(). Hearing Preservation Following Repeated Adenovector Delivery. Anat Rec (Hoboken).
Applications
Undefined
(). Regulation of Sema3c and the interaction between cardiac neural crest and second heart field during outflow tract development. Sci Rep.
Applications
IHC, ICC, Histology; Multiplex Assay
(). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis Cell Stem Cell.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
(). Differential roles of NF-Y transcription factor in ER chaperone expression and neuronal maintenance in the CNS. Sci Rep.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). CCAAT/enhancer-binding protein β regulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes. J Biol Chem
Applications
IHC, ICC, Histology
(). NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat Commun.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology; Multiplex Assay
(). Bladder cancers arise from distinct urothelial sub-populations. Nat Cell Biol.
Applications
IHC, ICC, Histology; Multiplex Assay
(). Simultaneous activation of Kras and inactivation of p53 induces soft tissue sarcoma and bladder urothelial hyperplasia. PLoS One
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Loss of aPKCλ in differentiated neurons disrupts the polarity complex but does not induce obvious neuronal loss or disorientation in mouse brains. PLoS One
Applications
IHC, ICC, Histology
(). N-terminal ataxin-3 causes neurological symptoms with inclusions, endoplasmic reticulum stress and ribosomal dislocation. Brain.
Applications
IHC, ICC, Histology
(). ERRγ regulates cardiac, gastric, and renal potassium homeostasis. Mol Endocrinol.
Applications
IHC, ICC, Histology
(). Deletion of voltage-gated channel affects glomerular refinement and odorant receptor expression in the mouse olfactory system. J Comp Neurol.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Induction of neuregulin signaling in mouse schwann cells in vivo mimics responses to denervation. J Neurosci
Applications
IF, Confocal Microscopy
(). Conditional overexpression of transgenes in megakaryocytes and platelets in vivo. Blood
Applications
IF, Confocal Microscopy; WB, IB, PCA
(). Peptide mass fingerprinting by matrix‐assisted laser desorption ionization mass spectrometry of proteins detected by immunostaining on nitrocellulose. Proteomics.
Applications
WB, IB, PCA
(). Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic‐like stem cells. Anat Rec A Discov Mol Cell Evol Biol.
Applications
IF, Confocal Microscopy; Multiplex Assay

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related Resources

GFP Antibodies: A Comprehensive Guide

Articles
View Article

Custom Recombinant Antibodies (rAbs)

Learn More

Custom Antibody Production

Learn More