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Primary Antibodies  >  Infectious Disease Antibodies
 

CRASP-2 Antibody

Rabbit Polyclonal
Code

200-401-C19
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot showing detection of 0.1 µg of recombinant CRASP-2 protein.  Lane 1: Molecular weight markers.  Lane 2:  MBP-CRASP-2 fusion protein (arrow; expected MW = 67.8 kDa).  Lane 3:  MBP alone.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min.  HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: Borrelia burgdorferi CRASP-2

CRASP-2 Antibody Properties

Description
Anti-CRASP-2 (RABBIT) Antibody - 200-401-C19
Host
Rabbit
Target Species
Borrelia burgdorferi
Known Cross Reactivity
Borrelia burgdorferi
Clonality
Polyclonal
Application
ELISA : 1:2,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
cspZ, BB_H06
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Lot#  

CRASP-2 Antibody Description

Background
CRASP-2 (Complement Regulator-Acquiring Surface Protein 2) of Borrelia burgdorferi binds FHL-1 and factor H binding protein in a distinct way. It may be predominantly expressed by serum-resistant Borrelia strains. Borrelia burgdorferi sensu lato has the ability to evade immune systems to persist in a variety of vertebrate hosts. This activity is dependent on a number of factors. Some Borrelia species bind host-derived fluid-phase immune regulators FHL-1 and factor H to their surface via complement regulator-acquiring surface proteins (CRASPs). Factor H and FHL-1 serve as cofactors for factor I, a serine protease that cleaves complement component 3b (C3b) directly on the cell surface and thereby confers resistance of spirochetes to complement-mediated lysis. It is possible that because of discontinuous binding regions in the factor H/FHL-1, long distance interaction may be involved in binding of both immune regulators. Putative coiled-coil structural elements may be important in the interaction of B. burgdorferi CRASP-1 with factor H.
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-2 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 25.4 kDa in size corresponding to Borrelia burgdorferi CRASP-2 protein by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi CRASP-2 protein. A BLAST analysis was used to suggest reactivity with CRASP-2 from B. burgdorferi sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. garinii, B. spielmanii, and valaisiana sources based on 91-89% homology. Cross-reactivity with CRASP-2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Rossmann E, Kitiratschky V, Hofmann H, Kraiczy P, Simon MM, and Wallich R. (2006) Borrelia burgdorferi Complement Regulator-Acquiring Surface Protein 1 of the Lyme Disease spirochetes is expressed in humans and induces antibody responses restricted to nondenatured structural determinants. Infection and Immunity, December 74:12, 7024-7028 Kraiczya P, Schreibera J, Skerkab C, Hauptb K, Bradea V, Wallich R, and Zipfel PF. (2008) Assessment of the regions within complement regulator-acquiring surface protein (CRASP)-2 of Borrelia burgdorferi required for interaction with host immune regulators FHL-1 and factor H. Proceedings IX. International Jena Symposium on Tick-borne Diseases (formerly IPS) March 15-17, 2007, International Journal of Medical Microbiology, 298:1, 268-271
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-301

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-319

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
100-4164P

Product Type
Primary Antibodies;
Applications
ELISA, WB
Catalog Number
K-711

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry