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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

CCL2 Antibody

Rabbit Polyclonal
Code

200-401-B60
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kDa (arrow) raised in yeast.  Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane.   Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Inc. Anti-bovine CCL2.   After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (611-743-127).
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Synonyms: Chemokine (C-C motif) ligand 2, monocyte chemotactic (or chemoattractant) protein-1 (MCP-1)

CCL2 Antibody Properties

Description
Anti-Bovine CCL2 (RABBIT) Antibody - 200-401-B60
Host
Rabbit
Target Species
Bovine
Known Cross Reactivity
bovine, swine, human, cat, orangutan, dog, mouse
Clonality
Polyclonal
Application
ELISA : 1:4,500
Western Blot : 1 µg/ml
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CCL2
Download CofA
Lot#  

CCL2 Antibody Description

Background
CCL2 is a small cytokine belonging to the CC chemokine family that recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing a signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a total molecular weight of approximately 8.8 kDa. The cell surface receptors that bind CCL2 are CCR2 and CCR4. CCL2 is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B (NF?B). In human osteoclasts, CCL2 and RANTES (regulated on activation normal T cell expressed and secreted) may act as an autocrine loop in human osteoclast differentiation. CCL2 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines.
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kDa in size corresponding to bovine CCL2 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine CCL2 protein. A BLAST analysis was used to suggest cross-reactivity with CCL2 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against swine based on 98% homology and human and cat (90%), orangutan and dog (89%) and mouse 90%). Cross-reactivity with CCL2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Liptak,A.R., Sullivan,B.T., Henkes,L.E., Wijayagunawardane,M.P., Miyamoto,A., Davis,J.S., Rueda,B.R. and Townson,D.H. (2005). Cooperative expression of monocyte chemoattractant protein 1 within the bovine corpus luteum: evidence of immune cell-endothelial cell interactions in a coculture system Biol. Reprod. 72 (5), 1169-1176 PMID 15659710 Xu LL, Warren MK, Rose WL, Gong W, Wang JM (01 September 1996). Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro. J. Leukoc. Biol. 60 (3): 365–71. Craig MJ, Loberg RD (December 2006). CCL2 (Monocyte Chemoattractant Protein-1) in cancer bone metastases. Cancer Metastasis Rev. 25 (4): 611–9. doi:10.1007/s10555-006-9027-x. PMID 17160712.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
bovine, swine, horse
Catalog Number
200-401-B53

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
bovine, swine, human, cat, orangutan, dog, mouse
Catalog Number
200-401-B60S

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bovine
Catalog Number
200-401-B74

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bovine
Catalog Number
201-401-B77

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry