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Primary Antibodies  >  Transcription Factor Antibodies
 

STAT1 phospho Y701 Antibody

Rabbit Polyclonal
Code

200-401-A43
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using  Rockland's Protein A purified anti-Stat1 pY701 antibody shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-??(lane 2).  No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1).  The membrane was probed with the primary antibody at a 1:1,000 dilution at 4° C, overnight.  Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
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Synonyms: DKFZp686B04100 antibody, ISGF 3 antibody, Signal transducer and activator of transcription 1 91kDa antibody, Signal transducer and activator of transcription 1 alpha/beta antibody, Signal transducer and activator of transcription 1 antibody

STAT1 phospho Y701 Antibody Properties

Description
Anti-STAT1 pY701 (RABBIT) Antibody - 200-401-A43
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:25,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
2.3 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
STAT1
Download CofA
Lot#  

STAT1 phospho Y701 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Signal transducer and activator of transcription 1 (Stat1) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor.  Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding. Stat1 has two isoforms,
Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Purity/Specifity
This product was Protein A purified from monospecific antiserum.  This antibody is specific for mouse Stat1 protein phosphorylated at Y701.  A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence.  Partial reactivity is expected against Stat1 from human sources as 90% homology is noted.  Cross-reactivity with Stat1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
100-401-861

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-A45

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse
Catalog Number
200-301-B65

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry