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Primary Antibodies  >  Neuroscience Antibodies
 

Beta-Site App-Cleaving Enzyme Antibody

Rabbit Polyclonal
Code

200-401-984
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunocytochemistry of BACE in 3T3 cells with BACE antibody at 10 µg/ml.
Western blot analysis of BACE in human brain tissue lysate in the absence (A) or presence (B) of blocking peptide, and in mouse 3T3 cell lysate (C) with BACE antibody at 1 µg/ml.
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$20.00
Synonyms: APP beta secretase antibody, Asp 2 antibody, ASP2 antibody, Aspartyl protease 2 antibody, BACE 1 antibody, BACE antibody, Beta secretase 1 antibody, Beta secretase antibody

Beta-Site App-Cleaving Enzyme Antibody Properties

Description
Anti-Beta-site APP-Cleaving Enzyme (BACE/Asp2) (RABBIT) Antibody - 200-401-984
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 0.5-1 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BASE1
Download CofA
Lot#  

Beta-Site App-Cleaving Enzyme Antibody Description

Background
Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer’s disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP. Anti-BACE antibody is ideal for investigators involved in Neuroscience research.
Immunogen
BACE Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human BACE protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
BACE antibody has been tested for use in ELISA, western blotting and immunohistochemistry. Human brain tissue can be used as a positive control in western blotting, and a band at approximately 70 kDa is expected.  Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-BACE Antibody is directed against human BACE protein.  The product was purified from monospecific antiserum by DEAE ion exchange chromatography.  The antibody is human and mouse reactive.  Reactivity against homologues from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Vassar R, Bennett BD, Babu-Khan S, et al. (1999) Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 286:735-741. Hussain I, Powell D, Howlett DR, et al. (1999) Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol. Cell. Neurosci. 14:419-427. Yan R, Bienkowski MJ, Shuck ME, et al. (1999) Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 402:533-537. Sinha S, Anderson JP, Barbour R, et al. ( 1999) Purification and cloning of amyloid precursor protein β-secretase from human brain. Nature 402:537-40.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Kits;
Reacts With
n/a
Catalog Number
KCB003

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry