Datasheet

NF-Y Antibody

Name: NF-Y Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

2 References

Item No. 200-401-101

Size: 100 µg

Applications: WB, ELISA, EMSA, IP

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

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Background

Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

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Specifications for NF-Y Antibody

Product Details

Description: Anti-NF-Y (B subunit) (RABBIT) Antibody - 200-401-101

Synonyms: rabbit anti-NF-Y Antibody, Nuclear transcription factor Y subunit B

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: NFYB - View All NFYB Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Purity/Specificity: This product was prepared from monospecific antiserum by a multi-step procedure which includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.

Database Links:

NCBI - P25208.2

UniProtKB - P25208

GeneID - 4801

Application Details

Tested Applications: ELISA, WB

Suggested Applications: EMSA, IP - View References

Application Note: This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific). Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 µl per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#611-1302) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate (#ABTS-100). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Grimm M et al. (2002). Inhibition of Major Histocompatibility Complex Class II Gene Transcription by Nitric Oxide and Antioxidants. J Biol Chem.

Applications

WB, IB, PCA

PubMed

Taira T et al. (1999). Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y. J Biol Chem.

Applications

IP, Co-IP

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Gel Shift Assay Protocol

Histone Immunoblotting Protocol

Immunoprecipitation (IP) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.