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GFP and Epitopes  >  Antibodies to FLAG™ and Antibodies to 6XHIS Tags
 

Anti-DYKDDDDK (FLAG® tag) Affinity Gel

Mouse Monoclonal 29E4.G7 IgG2a kappa
Code

200-350-383
Size

1 mL
Price

$279.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel.
Lane 1:  Cell lysate before purification. 
Lane 2:  Flow through (used cell lysate).    Lane 3:  Purified DYKDDDDK (FLAG® tag)  recombinant protein (arrowhead).
Load:  (6 µL per lane). 
Predicted/Observed size:  70kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel.
Lane 1:  crude lysate containing over-expressed DYKDDDK-tagged recombinant protein .  
Lane 2:  unbound flow-through of consisting of endogenous E.coli proteins.  Lane 3:  enriched recombinant protein.  
Load:  5 µg protein, 15µL flow through.
Predicted/Observed size:  70kDa for DYKDDDDK tagged recombinant protein.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: FLAG® tag, DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K

Anti-DYKDDDDK (FLAG® tag) Affinity Gel Properties

Description
Anti-DYKDDDDK Affinity Gel (Binds the same epitope as Sigma's Anti-FLAG® M2 Antibody) - 200-350-383
Host
Mouse
Known Cross Reactivity
Internal, carboxy and amino terminal linked FLAG® tagged recombinant proteins
Clonality
Monoclonal 29E4.G7 IgG2a kappa
Application
ImmunoPrecipitation: 10 µL resin binds >1 µg FLAG® fusion protein
Epitope Tag Type
FLAG
Physical State
Suspension of agarose beads
Shipping condition
Wet Ice
Format
IgG2a
Label
Concentration
0.99 mg antibody per cc agarose
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

Anti-DYKDDDDK (FLAG® tag) Affinity Gel Description

Background
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means.  Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG® and HA.
Immunogen
Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Immunogen Type
Peptide
Storage Condition
Store vial at 4°C prior to opening.
Application Note
Anti-DYKDDDDK Affinity Gel is optimally suited for immunoprecipitation and purification of FLAG® tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG® epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Anti-FLAG® is a registered trademark of Sigma-Aldrich. Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.
Purity/Specifity
Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose. This product is intended for purification of proteins containing the FLAG® epitope tag sequence. Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG® epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) is recommended for competitive elution to recover fusion protein (see protocol).
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Chubet, R.G. and Brizzard, B.L. (1996) Vectors for expression and secretion of FLAG® epitope-tagged proteins in mammalian cells. Biotechniques 20(1):136-141. Slootstra, J.W., et al. (1997) Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG® monoclonal antibodies M1, M2 and M5. Mol. Divers. 2(3):156-164. Robeva, A.S., et al. (1996) Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG® epitope. Development of an efficient generic protein purification procedure. J. Biochem. Pharmacol. 51(4):545-555.
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, FC
Reacts With
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Catalog Number
200-301-383

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Reacts With
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Catalog Number
600-401-383

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Reacts With
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Catalog Number
600-403-383

Product Label

 

Description
Agarose
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry