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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP

GFP Antibody Dylight™ 488 Conjugated

Mouse Monoclonal 9F9.F9 IgG1 kappa


100 µg


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Multi-color Immunofluorescence Microscopy using DyLight™ dyes.  
Tissue:  human breast carcinoma.  
Fixation:  0.5% PFA. 
Antigen retrieval:  not required.
Primary antibody:  Anti-Histone and Anti-Tubulin antibody at 10 µg/mL  for 1 h at RT.
Secondary antibody:  DyLight™ 488 conjugate and DyLight™ 549 conjugate mouse secondary antibody at 1:10,000 for 45 min at RT.
Localization:  Histone is nuclear and Tubulin is cytoplasmic.
Staining:  Anti-Histone detection using a DyLight™ 488 conjugate (green) fluorescent signal and Anti-Tubulin was detected using a DyLight™ 549 conjugate (red) fluorescent signal.  Nuclei were counter-stained using DAPI (blue).
Western Blot of DyLight™ 488 conjugated monoclonal anti-GFP antibody to detect GFP control proteins.  
Lane 1:  His-Sumo-GFP.  
Lane: Molecular Weight.
Lane 2:  Beta-Galactosidase (negative control).  
Lane 3:  recombinant GFP control protein (000-001-215).
Load:  35 µg per lane.
Primary antibody:  none.
Secondary antibody:  DyLight™ 488 conjugated monoclonal anti-GFP (200-341-215) secondary antibody at 1:5,000. Block:  MB-070 for 2 hr at RT.
Predicted/Observed size:  27kDa/54kDa, 27kDa for rGFP/~45kDa His-Sumo-GFP. 
Other band(s): none.
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$40.00 to United States
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

GFP Antibody Dylight™ 488 Conjugated Properties

Anti-GFP (MOUSE) Monoclonal Antibody DyLight™ 488 Conjugated - 200-341-215
Known Cross Reactivity
wt, rGFP, eGFP
Monoclonal 9F9.F9 IgG1 kappa
ELISA : 1:20,000-1:40,000
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : 1:10,000 - 1:25,000
Other Dilution: User Optimized
Epitope Tag Type
Physical State
Shipping condition
FP Value
3.0 moles DyLight™ 488 per mole of IgG
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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GFP Antibody Dylight™ 488 Conjugated Description

GFP monoclonal antibody is Dylight™ 488 Conjugated. Labeled GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.
BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.  A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
GFP Dylight™ 488 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Catalog Number

Product Type
GFP and Epitopes;
Reacts With
wt, rGFP, eGFP
Catalog Number

Product Type
GFP and Epitopes;
Reacts With
wt, rGFP, eGFP
Catalog Number

Product Type
GFP and Epitopes;
Reacts With
wt, rGFP, eGFP
Catalog Number

Product Label


DyLight™ 488
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester