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Primary Antibodies > Sample Size Primary Antibodies

LGR4 Antibody

Mouse Monoclonal 6G8.B3.G5.C3 IgG2b kappa

Code

200-301-B45S

Size

25 µL

Price

$89.00

Availability

Ships next business day.

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Rockland's anti-LGR4 monoclonal antibody was used diluted to 5 ?g/ml to detect LGR4 staining at the membrane of cells in various human tissues. A. Brain cerebellum. B. Pancreas islet. Strongly positive staining is noted in subsets of cells within the islets of Langerhans. Moderately positive staiing was observed in Purkinje and Golgi neurons of the cerebellum, adrenal medulla, neuroendocrine cells, hepatocytes, lung macrophages, seminiferous tubules and Leydig cells of the testis. Faintly to moderately positive staining was also observed in cardiac myocytes and renal tubules, granulocytes, and subsets of lymphocytes. Some elastin background staining is noted. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.

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Synonyms: LGR4 leucine-rich repeat-containing G protein-coupled receptor 4

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LGR4 Antibody Properties

Description

Anti-LGR4 (MOUSE) Monoclonal Antibody - 200-301-B45S

Host

Mouse

Target Species

Human

Known Cross Reactivity

human, chimpanzee, macaque

Clonality

Monoclonal 6G8.B3.G5.C3 IgG2b kappa

Application

ELISA : 1:20,000 - 1:100,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 5 µg/mL
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.13 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

LGR4

LGR4 Antibody Description

Background

LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Application Note

This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Purity/Specifity

This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human LGR4 protein. A BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Weng,J., Luo,J., Cheng,X., Jin,C., Zhou,X., Qu,J., Tu,L., Ai,D.,Li,D., Wang,J., Martin,J.F., Amendt,B.A. and Liu,M.(2008) Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2. Proc. Natl. Acad. Sci. U.S.A. 105 (16), 6081-6086. Gao,Y., Kitagawa,K., Hiramatsu,Y., Kikuchi,H., Isobe,T., Shimada,M., Uchida,C., Hattori,T., Oda,T., Nakayama,K., Nakayama,K.I., Tanaka,T., Konno,H. and Kitagawa,M. (2006) Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis. Cancer Res. 66 (24), 11623-11631. Loh,E.D., Broussard,S.R. and Kolakowski,L.F. (2001) Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor. Biochem. Biophys. Res. Commun. 282 (3), 757-764. Loh,E.D., Broussard,S.R., Liu,Q., Copeland,N.G., Gilbert,D.J., Jenkins,N.A. and Kolakowski,L.F. Jr. (2000) Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping. Cytogenet. Cell Genet. 89 (1-2), 2-5. Hsu,S.Y., Liang,S.G. and Hsueh,A.J. (1998) Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region. Mol. Endocrinol. 12 (12), 1830-1845.

Specific Reference

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Beta Galactosidase Conjugates

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Biotin Conjugates

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