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Primary Antibodies  >  Sample Size Primary Antibodies
 

STAT5 phospho Y694 Antibody

Mouse Monoclonal 5F6.F1 IgG1 kappa
Code

200-301-A45S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Immunohistochemistry using Rockland's Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5 pY694 in human breast tissue (40X).  The antibody was used a dilution to 20 µg/mL.   The image shows breast epithelium with moderate nuclear staining.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
Western blot using Rockland's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2).  No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1).   The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4° C.  For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody

STAT5 phospho Y694 Antibody Properties

Description
Anti-STAT5 pY694 (MOUSE) Monoclonal Antibody - 200-301-A45S
Host
Mouse
Target Species
Mouse
Known Cross Reactivity
human, mouse, rat
Clonality
Monoclonal 5F6.F1 IgG1 kappa
Application
ELISA : 1:20,000
IF Microscopy : 1:50 - 1:1,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 20-40 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
STAT5A
Download CofA
Lot#  

STAT5 phospho Y694 Antibody Description

Background
Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5??and Stat5?. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.
Immunogen
Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 91 kDa in size corresponding
Purity/Specifity
Phospho STAT5 pY694 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694.  A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence.  Cross-reactivity with Stat5a from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Engblom,D., Kornfeld,J.W., Schwake,L., Tronche,F., Reimann,A., Beug,H., Hennighausen,L., Moriggl,R. and Schutz,G. (2007) Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression. Genes Dev. 21 (10), 1157-1162. Baugh,J.E. Jr., Floyd,Z.E. and Stephens,J.M. (2007) The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes. Obesity (Silver Spring) 15 (3), 583-590. Laurence,A., Tato,C.M., Davidson,T.S., Kanno,Y., Chen,Z., Yao,Z., Blank,R.B., Meylan,F., Siegel,R., Hennighausen,L., Shevach,E.M. and O'Shea,J.J. (2007) Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation. Immunity 26 (3), 371-381.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
100-401-861

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry