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Primary Antibodies  >  Sample Size Primary Antibodies
 

Wnt1 Antibody

Mouse Monoclonal 13F9 IgG1 kappa
Code

200-301-A37S
Size

25 µL
Price

$95.00
Availability

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Western blot using Rockland's protein-A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate.   The results show specific binding corresponding to the ~41 kDa Wnt1 protein.  Primary antibody was used at a 1:500 dilution.  Personal communication,  Stephen Brown, Brown University
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Synonyms: INT1, Proto-oncogene protein Wnt-1

Wnt1 Antibody Properties

Description
Anti-Wnt1 (MOUSE) Monoclonal Antibody - 200-301-A37S
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal 13F9 IgG1 kappa
Application
ELISA : 1:4,000 - 1:20,000
Western Blot : 1:175 - 1:250
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
WNT1
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Lot#  

Wnt1 Antibody Description

Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.
Immunogen
Synthetic peptide corresponding to an internal region of human Wnt1 protein
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 41 kDa in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with human and mouse Wnt1 protein.  A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence.  Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology.  Cross-reactivity with Wnt1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
De Ferrari,G.V. and Moon,R.T. (2006) The ups and downs of Wnt signaling in prevalent neurological disorders. Oncogene 25 (57), 7545-7553. Yook,J.I., Li,X.Y., Ota,I., Hu,C., Kim,H.S., Kim,N.H., Cha,S.Y., Ryu,J.K., Choi,Y.J., Kim,J., Fearon,E.R. and Weiss,S.J. (2006) A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells.  Nat. Cell Biol. 8 (12), 1398-1406. He,J., Sheng,T., Stelter,A.A., Li,C., Zhang,X., Sinha,M., Luxon,B.A. and Xie,J.  (2006) Suppressing Wnt signaling by the hedgehog pathway through sFRP-1.  J. Biol. Chem. 281 (47), 35598-35602.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, human
Catalog Number
100-401-223

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-424

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-429

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-444

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry