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Primary Antibodies  >  Phospho Specific Antibodies
 

SMC1 phospho S957 Antibody

Mouse Monoclonal 5D11.G5 IgG1 kappa
Code

200-301-397
Size

100 µg
Price

$355.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Rockland's Protein G Purified Mab anti-SMC1 pS957 antibody was used at a 2.5 µg/ml to detect nuclear signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong nuclear anti-SMC1 pS957 staining of human breast ductal epithelium. Tissue was formalin-fixed and paraffin embedded.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.  Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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Shipping:
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Synonyms: Structural maintenance of chromosomes protein 1B antibody, SMC1beta protein antibodySMC1B antibody, SMC1L2 antibody

SMC1 phospho S957 Antibody Properties

Description
Anti-SMC1 pS957 (MOUSE) Monoclonal Antibody - 200-301-397
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Monoclonal 5D11.G5 IgG1 kappa
Application
ELISA : 1:20,000 - 1:100,000
IF Microscopy : 2.5 µg/ml
Western Blot : 1:100 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SMC1B
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Lot#  

SMC1 phospho S957 Antibody Description

Background
Structural maintenance of chromosomes (SMC) proteins play important roles in sister chromatid cohesion, chromosome condensation, sex-chromosome dosage compensation, and DNA recombination and repair (DNA damage). Protein complexes containing heterodimers of the SMC1 and SMC3 proteins have been implicated specifically in both sister chromatid cohesion and DNA recombination.  ATM, a protein kinase belonging to the phosphatidylinositol 3-kinase family that regulates cell cycle checkpoints and DNA recombination and repair, phosphorylates SMC1 protein after ionizing irradiation. ATM protein kinase phosphorylates SMC1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint.  Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM protein kinase substrates NBS1 and BRCA1. These same sites in SMC1 are phosphorylated after treatment with UV irradiation or hydroxyurea in an ATM-independent manner, thus demonstrating that another kinase must be involved in responses to these cellular stresses. Yeast containing hypomorphic mutations in SMC1 and human cells overexpressing SMC1 mutated at both of these phosphorylation sites exhibit decreased survival following ionizing irradiation. These results demonstrate that SMC1 participates in cellular responses to DNA damage and link SMC1 to the ATM protein kinase signal transduction pathway.
Immunogen
This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Protein G Purified Mab anti-SMC1 was tested by ELISA, immunohistochemistry and western blotting against native protein. The antibody reacts with SMC1 from irradiated human and mouse cells.  A 160 kDa band corresponding to human SMC1 is noted in
Purity/Specifity
This Protein G Purified Mab antibody is directed against human SMC1 and is useful in determining its presence in various assays. This monoclonal anti-SMC1 antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.  Minimal reactivity is observed against the non-phosphorylated epitope.  Reactivity is observed against human and mouse SMC1.  Cross reactivity with SMC1 from other eukaryotic sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Seong-Tae Kim, Bo Xu, and Michael B. Kastan (2002) Involvement of the cohesin protein, Smc1, in ATM-dependent and independent responses to DNA damage. Genes Dev. March; 16 (5): 560–570. Bakkenist, C. J. & Kastan, M. B. (2003). DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 421, 499-506.
Specific Reference
Kitagawa, R., Bakkenist, C.J., McKinnon, P.J. and Kastan, M.B. (2004) Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 18(12):1423-1438. Callén E, Jankovic M, Wong N, Zha S, Chen HT, Difilippantonio S, Di Virgilio M, Heidkamp G, Alt FW, Nussenzweig A, Nussenzweig M. (2009) Essential Role for DNA-PKcs in DNA Double-Strand Break Repair and Apoptosis in ATM-Deficient Lymphocytes. Mol Cell. 34(3):285-97.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
mouse
Catalog Number
600-401-927


Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry