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Primary Antibodies  >  Cancer Research Antibodies

HSP27 Antibody

Mouse Monoclonal G3.1 IgG1 kappa


100 µg


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Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot.  Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane.    The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using IRDye™800  labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000.  A single band corresponding to human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown).  The antibody may be used to detect endogenous human HSP27.  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
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Synonyms: Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody

HSP27 Antibody Properties

Anti-HSP27 (MOUSE) Monoclonal Antibody - 200-301-243
Target Species
Known Cross Reactivity
Monoclonal G3.1 IgG1 kappa
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
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HSP27 Antibody Description

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in different cell types and tissues (this protein has also been referred to as the Estrogen-Regulated 24 kDa protein, hsp25 and hsp28).   Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and post-translational level.  In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change.  Human HSP27 shares greater than 50% homology with the low molecular weight Drosophila HSP and mammalian crystalline lens protein.  Because of the estrogen responsive nature of HSP27, the protein has been extensively
This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting, immunoprecipitation, ELISA, immunohistochemistry and immunocytochemistry.  The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma.    Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry.
This protein A purified mouse monoclonal antibody reacts specifically with HSP27 in human tissues and cell lines.  MCF-7 cells are recommended as a positive control.  Cross reactivity with hsp27 from other mammalian sources is likely.  No cross reactivity occurs with HSP70, HSP90 or HSP104.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Ines Moretti-Rojas, et. al. (1988) A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Breast Cancer Res. and Treat., 11: 155-163. McGuire, S.E. et. al. (1989) Chromasomal Assignment of Human 27-kDa Heat Shock Protein Gene Family. Somatic Cell and Molecular Genetics, 15: 167-171. Edwards, D.P., et. al. (1981) Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7). Breast Cancer Res. Treat., 1: 209-223.
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Proteins and Peptides;
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Primary Antibodies;
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human, mouse, rat, monkey
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