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GFP and Epitopes  >  Antibodies to GST
 

GST Antibody

Mouse Monoclonal 3D4 IgG1 kappa
Code

200-301-200
Size

500 µg
Price

$295.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Mouse-anti-GST antibody.  
Lane 1:  molecular weight.  
Lane 2:  Mouse-anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha-1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST.  
Load:  1 µg per lane.
Primary antibody:  Each primary antibody at 1:1000 for overnight at 4°C.
Secondary antibody:   DyLight549 Donkey anti-Rabbit IgG (green)   DyLight 488 Donkey anti-Mouse IgG (blue),  and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT.
Block:  MB-070 overnight at 4°C.
Western Blot of anti-GST antibody.  
Lane 1:  GST recombinant protein.  
Lane 2:  lysate of HeLa cells expressing recombinant GST protein.  
Load:  0.1 µg per lane.
Primary antibody:  GST antibody at  1.0µg/ml for 1 h at room temperature.
Secondary antibody:  IRDye800™ goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: GST, Glutathione-S-Transferase

GST Antibody Properties

Description
Anti-GST (MOUSE) Monoclonal Antibody - 200-301-200
Host
Mouse
Clonality
Monoclonal 3D4 IgG1 kappa
Application
ELISA : 1:100,000 - 1:900,000
Western Blot : 1:1,000 - 1:10,000
Immunochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Epitope Tag Type
GST
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA
Lot#  

GST Antibody Description

Background
Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Immunogen
A BALB/c mouse was immunized with Glutathione-S-Transferase [Schistosoma japonicum].
Immunogen Type
Native Protein
Storage Condition
Store anti-GST at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency
Purity/Specifity
GST monoclonal antibody is a protein A purified mouse monoclonal which reacts specifically with GST.  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Epstein, Alan L. and Clevenger, Charles V.: Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immunobiochemical methods using monoclonal antibodies. In : Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp. 117-137. Smith D.B., Davern K.M., Board P.G., Tiu W.U., Garcia E.G., Mitchell G.F. (1986) Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proc. Natl. Acad. Sci. U.S.A. 83:8703-8707.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry