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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

Cdc2 p34 Antibody

Mouse Monoclonal POH-1 IgG2a kappa
Code

200-301-160
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Mab anti-Human p34cdc2 antibody (clone POH-1) is shown to detect human p34cdc2by western blot.  Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane.    The blot was incubated with a 1:1,000 dilution of Mab anti-Human p34cdc2at room temperature for 30 min followed by detection using IRDye™800  labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000.  A doublet band corresponding to human p34cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown).  The antibody may be used to detect endogenous human p34cdc2.  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
Mab anti-Human p34cdc2 antibody was used to detect human p34cdc2by western blot in untreated (control) and drug treated (10 µM genistein) lysates of MCF-7 cells.  Very strong detection occurs using a 1:1,000 dilution.  Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
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Synonyms: Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 antibody, p34 Cdk1 antibody, p34 protein kinase antibody

Cdc2 p34 Antibody Properties

Description
Anti-cdc2 (p34) (MOUSE) Monoclonal Antibody - 200-301-160
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Monoclonal POH-1 IgG2a kappa
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG2a
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDC2
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Lot#  

Cdc2 p34 Antibody Description

Background
p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF).  The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues.  In humans the phosphor-ylation appears to be performed by p60src.  The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins.  The phosphorylation activity is coupled to the entry into the M-phase of the cell.  p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally.  Association with deletion mutants of cyclin halts the M-phase before it is completed.
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase),  and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control.   Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method.
Purity/Specifity
This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines.  This antibody is not cross reactive with other cyclin dependent kinases.  Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur.  This reagent has broad interspecies reactivity.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Lukas, J. et al. (1992) Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem., 207, 169-176. Ye, X.S. et al. (1997) Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. J. Biol Chem 272(52):33384-93. Palmer, A. et al. (1998) A link between MAP kinase and p34cdc2/cyclin B during oocyte maturation: p90rsk phosphorylates the p34cdc2 inhibitory kinase Myt1. EMBO J. 17, 5037-5047.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat, mouse
Catalog Number
100-401-152

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, ChIP
Reacts With
human
Catalog Number
200-301-174

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry