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SIX3 Antibody

Guinea Pig Polyclonal
NCI Collaboration
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Immunofluorescence microscopy using Rockland's Protein A purified anti-Six3 antibody shows detection of Six3 in various E14.5 mouse embryonic tissues.  Tissue was fixed using 4% PFA.  The primary antibody was used at a 1:200 dilution. Personal Communication, A. Lavado, St Jude Children's Research Hospital, Memphis, TN.
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Number:
200-201-A26S
Size:
25 µL
Price:
$95.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

SIX3 Antibody Properties

Description
Anti-Homeobox Protein SIX3 (GUINEA PIG) Antibody - 200-201-A26S
Host
Guinea Pig
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
IF Microscopy : 1:200 - 1:1,000
Western Blot : 1:1000
Immunohistochemistry: 1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
0.65 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SIX3
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SIX3 Antibody Description

Background
Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke’s pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.
Synonyms
Six3, Sine oculis homeobox homolog 3
Immunogen
This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This Protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was purified by Protein A chromatography from monospecific antiserum.  This antibody reacts with mouse Six3.   Expect partial reactivity with Six3 from chicken and Xenopus sources based on 95% and 85% homologies, respectively.  Cross-reactivity with Six3 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Liu W, Lagutin OV, Mende M, Streit A, Oliver G.  (2006) Six3 activation of Pax6 expression is essential for mammalian lens induction and specification. EMBO J. 25(22):5383-95. Oliver,G., Mailhos,A., Wehr,R., Copeland,N.G., Jenkins,N.A. and Gruss,P. (1995) Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development.  Development 121 (12), 4045-4055. Kawakami,K., Ohto,H., Takizawa,T. and Saito,T. (1996) Identification and expression of six family genes in mouse retina.  FEBS Lett. 393 (2-3), 259-263.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
mouse
Catalog Number
200-201-A26

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-301-A37

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-301-A37S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
mouse
Catalog Number
600-401-A26

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry