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Peroxidase Antibody

Goat Polyclonal
NCI Collaboration
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Rockland Goat anti Peroxidase antibody was used to detect horse radish peroxidase under reducing and non-reducing conditions. Reduced samples of purified horse radish peroxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE.  Protein was transferred to nitrocellulose and probed with Rockland Goat anti Peroxidase antibody (200-1138 lot 25614 1:5K in MB-0070, ON 4 C).  Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 lot 20834 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.
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Number:
200-101-038
Size:
100 µg
Price:
$295.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Peroxidase Antibody Properties

Description
Anti-PEROXIDASE (Horseradish) (GOAT) Antibody - 200-101-038
Host
Goat
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PRXC, HRPC
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Peroxidase Antibody Description

Immunogen
Peroxidase (Horseradish)
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Peroxidase (Horseradish).  Cross-reactivity against Peroxidase from other tissues and species may occur but has not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
FLISA, WB
Catalog Number
605-431-013

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Catalog Number
605-441-013

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Catalog Number
605-451-013

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry