House Phospho Banner
 
You may also want to consider these related products.
Anti-Human IL-1 Receptor Type I (IL-1RI) (RABBIT) Antibody - 109-401-304
Anti-Human IL-1 Receptor Type I (IL-1RI) (RABBIT) Antibody - 109-401-304
Anti-Human IL-1 Receptor Antagonist (RABBIT) Antibody - 209-401-303
Anti-Human IL-1 Receptor Antagonist (RABBIT) Antibody - 209-401-303
Anti-Human IL-1 Receptor Type II (IL-1RII) (RABBIT) Antibody - 209-401-305
Anti-Human IL-1 Receptor Type II (IL-1RII) (RABBIT) Antibody - 209-401-305
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-1 Receptor Type IL Antibody

Rabbit Polyclonal
Code

109-401-305
Size

1 mL
Price

$355.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western blot using Rockland's anti-IL-1RII antibody.  This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown).  Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.  After blocking, the membrane was probed with the primary antibody diluted to 1:1,000.  Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (611-132-122) for 45 min at room temperature.  Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel).  IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: IL1RB antibody, Interleukin 1 receptor type II precursor antibody, MGC47725 antibody, p68 antibody, Type II interleukin 1 receptor beta antibody, Antigen CDw121b antibody

IL-1 Receptor Type IL Antibody Properties

Description
Anti-Human IL-1 Receptor Type II (IL-1RII) (RABBIT) Antibody - 109-401-305
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, primate
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:2,000
Western Blot : 1:500 - 1:2,000
ImmunoPrecipitation: 1:400 - 1:800
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL1R2
Download CofA
Lot#  

IL-1 Receptor Type IL Antibody Description

Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antiserum against Human IL-1 Receptor Type II (IL-1RII) has been tested for use in ELISA, radioimmunoassays and immunoprecipitation.  Reactivity in other immunoassays is unknown.  IL-1RII has been termed p68 or CD121 in some laboratories.  IL-1RII has been shown to bind IL-1, IL-1a and IL-1b.
Purity/Specifity
This antiserum is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells.  In general, this antibody also detects primate IL-1RII.  The antibody does not recognize human IL-1, IL-1Ra or IL-1R type I.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Arend, W.P., Malyak, M., et al. (1994) Binding of IL-1?, IL-1?, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids. J. Immunol. 153: 4766-4774. Colotta, F., Dower, S.K., et al. (1994) The type II decoy receptor: a novel regulatory pathway for interleukin-1. Immunol. Today 15: 562-566. Colotta, F., Re, F., et al. (1993) Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4. Science 261: 472-475
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, Neu, FC
Reacts With
human, primate
Catalog Number
109-401-304

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-303

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-305

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry