Biotin Antibody
Western Blot of Anti-Biotin Antibody
Western Blot of Anti-Biotin antibody
Anti-Biotin Antibody
Anti-Biotin Antibody
Anti-Biotin Antibody
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Datasheet

Biotin Antibody

Rabbit Polyclonal

23 References
100-4198
2 mL
WB, ELISA, IF, EM, Multiplex, Other
Biotin
Rabbit
$426.00 /Per Item
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Background

Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

Product Details

Anti-Biotin (RABBIT) Antibody - 100-4198
rabbit anti-biotin antibody, rabbit anti biotin
Rabbit
Polyclonal
Antiserum

Target Details

Biotin
Native Protein
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
This product was prepared from monospecific antiserum by a delipidation and defibrination.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, biotinylated IgG and Biotinylated BSA.

Application Details

ELISA
EM, IF, Other, WB, Multiplex  - View References
Anti-Biotin has been tested in ELISA and is suitable for immunoblotting (western or dot blot), immunoprecipitation and most immunological methods requiring high titer and specificity.

Formulation

Lyophilized
90 mg/mL by Refractometry
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages. Sci Rep.
Applications
IF, Confocal Microscopy; TEM
(). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytol.
Applications
Immuno-EM
(). A role for Rab30 in retrograde trafficking and maintenance of endosome-TGN organization. Exp Cell Res.
Applications
Undefined
(). The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes. Science.
Applications
Immuno-EM
(). SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport. EMBO J.
Applications
Immuno-EM
(). Human VAPome Analysis Reveals MOSPD1 and MOSPD3 as Membrane Contact Site Proteins Interacting with FFAT-Related FFNT Motifs. Cell Rep.
Applications
Immuno-EM
(). Micro–nanoparticles magnetic trap: Toward high sensitivity and rapid microfluidic continuous flow enzyme immunoassay. Biomicrofluidics.
Applications
Other
(). SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals. Nat Commun.
Applications
Immuno-EM
(). Limiting Neuronal Nogo Receptor 1 Signaling during Experimental Autoimmune Encephalomyelitis Preserves Axonal Transport and Abrogates Inflammatory Demyelination. J Neurosci.
Applications
Immuno-EM
(). The ubiquitin‐conjugating enzyme UBE 2 QL 1 coordinates lysophagy in response to endolysosomal damage. EMBO Rep.
Applications
Immuno-EM
(). Pseudomonas aeruginosa lectin LecB impairs keratinocyte fitness by abrogating growth factor signalling. Life Sci Alliance.
Applications
Immuno-EM
(). BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis. Science.
Applications
Immuno-EM
(). Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish. Nat Neurosci.
Applications
Immuno-EM
(). Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathologica
Applications
Immuno-EM
(). A redox signalling globin is essential for reproduction in Caenorhabditis elegans. Nature Communications
Applications
TEM
(). Immuno correlative light and electron microscopy on Tokuyasu cryosections. Methods Cell Biol.
Applications
IF, Confocal Microscopy; Immuno-EM
(). Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex. RNA.
Applications
Immuno-EM
(). Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways. PLoS Pathog.
Applications
WB, IB, PCA
(). Detecting protein-ligand binding on supported bilayers by local pH modulation. Journal of the American Chemical Society
Applications
EIA, ELISA
(). Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation. Cell.
Applications
WB, IB, PCA
(). Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins. J Cell Biol.
Applications
WB, IB, PCA
(). Concentration of rafts in platelet filopodia correlates with recruitment of c‐Src and CD63 to these domains. J Thromb Haemost.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). One-step direct assay for mature-type adrenomedullin with monoclonal antibodies. Clin Chem.
Applications
Other

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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