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Datasheet SDS

Xanthine Oxidase Antibody

Rabbit Polyclonal

3 References
100-4183
2 mL
WB, ELISA, IF, Multiplex
Bovine
Rabbit
$1,225.00 /Per Item
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Shipping info:

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Background

Xanthine dehydrogenase/oxidase is a key enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine. It catalyzes the oxidation of xanthine to uric acid. It contributes to the generation of reactive oxygen species. Xanthine dehydrogenase/oxidase can be converted from the dehydrogenase form (D) to the oxidase form (O) irreversibly by proteolysis or reversibly through the oxidation of sulfhydryl groups.

Product Details

Anti-Xanthine Oxidase (Buttermilk) (RABBIT) Antibody - 100-4183
rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase/oxidase, Xanthine dehydrogenase (XD), Xanthine oxidase (XO), Xanthine oxidoreductase
Rabbit
Polyclonal
Antiserum

Target Details

XDH  - View All XDH Products
Bovine
Native Protein
Xanthine Oxidase [Bovine Buttermilk]
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Application Details

ELISA
IF, WB, Multiplex  - View References
Xanthine oxidase antibody has been tested by ELISA. this product has been assayed against 1.0 ug of Xanthine Oxidase [Bovine Buttermilk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Formulation

Lyophilized
75 mg/mL by Refractometry
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Seasonal superoxide overproduction and endothelial activation in guinea-pig heart; seasonal oxidative stress in rats and humans. J Mol Cell Cardiol.
Applications
WB, IB, PCA
(). Contribution of xanthine oxidoreductase to mammary epithelial and breast cancer cell differentiation in part modulates inhibitor of differentiation-1. Molecular Cancer Research : Mcr
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Migratory activity of human breast cancer cells is modulated by differential expression of xanthine oxidoreductase. Journal of Cellular Biochemistry
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.