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GFP and Epitopes  >  Antibodies for Signal Enhancement
 

Peroxidase Antibody

Rabbit Polyclonal
Code

100-4138
Size

2 mL
Price

$185.00
Availability

Ships next business day.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Peroxidase antibody, anti-HRP, Peroxidase antibody raised in rabbit, rabbit anti-HRP, Rb-anti-HRP, Rb-anti-Peroxidase, Horseradish Peroxidase

Peroxidase Antibody Properties

Description
Anti-PEROXIDASE (Horseradish) (RABBIT) Antibody - 100-4138
Host
Rabbit
Known Cross Reactivity
pan reactive
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
90 mg/mL by Refractometry
Buffer
See application note.
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
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Lot#  

Peroxidase Antibody Description

Background
Anti-Peroxidase antibody is a valuable tool for most common immunoassays including immunoprecipitation (IP), ELISA, western blotting (WB) and immunohistochemistry (IHC).
Immunogen
Anti-Peroxidase antibody was produced by repeated immunization of rabbits with peroxidase isolated from horseradish.
Immunogen Type
Native Protein
Storage Condition
Store anti-HRP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Anti-Peroxidase antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. Note the buffer for this anti-peroxidase antibody is 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.6
Purity/Specifity
Anti-Peroxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
200-101-038

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
200-2138

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
200-3138

Product Type
GFP and Epitopes;
Applications
ELISA, WB
Catalog Number
200-5138

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry