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Primary Antibodies  >  Enzyme Antibodies
 

Ovalbumin Antibody

Rabbit Polyclonal
Code

100-4133
Size

2 mL
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Rabbit anti-Ovalbumin antibody.  
Lane 1:  ~1ug of purified Ovalbumin reduced.  
Lane 2:  ~ 0.25ug of purified Ovalbumin reduced.  
Lane 3:  ~1ug of purified Ovalbumin non-reduced.  
Lane 4:  ~0.25ug of purified Ovalbumin non-reduced.
Primary antibody:  anti Ovalbumin antibody at 1:5000 for overnight at 4°C.
Secondary antibody:  Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hours at RT.
Block:  MB-070 overnight at 4°C.
Predicted/Observed size:  42.9 kDa, ~36 kDa for Ovalbumin. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Ovalbumin, Egg albumin, Plakalbumin, Allergen Gal d II Allergen=Gal d 2

Ovalbumin Antibody Properties

Description
Anti-OVALBUMIN (Hen Egg White) (RABBIT) Antibody - 100-4133
Host
Rabbit
Target Species
Chicken
Known Cross Reactivity
chicken (Gallus gallus)
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SERPINB14
Download CofA
Lot#  

Ovalbumin Antibody Description

Background
Anti-Ovalbumin Antibody detects Ovalbumin. Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. It is a non-inhibitory serpin. It is the storage protein of egg white. Anti-Ovalbumin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.
Immunogen
Ovalbumin [Hen Egg White] Anti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Anti-ovalbumin Antibody is suitable for western blotting, ELISA and IP. Researchers should determine optimal titers for applications that are not stated below.
Purity/Specifity
Ovalbumin Antibody is an antiserum prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Ovalbumin [Hen Egg White].  Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Hu H.Y., Du H.N. (2000). "Alpha to Beta Structural Transformation of Ovalbumin: Heat and pH Effects". Journal of Protein Chemistry 19 (3): 177–183
Specific Reference
Wang, J., Zhang, Y., Wei, J., Zhang, X., Zhang, B., Zhu, Z., Zou, W., Wang, Y., Mou, Z., Ni, B. and Wu, Y. (2007), Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response. Immunology, 121: 174–182. doi: 10.1111/j.1365-2567.2007.02554.x
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
chicken
Catalog Number
100-4172

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry