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Primary Antibodies  >  Apoptosis Antibodies
 

Scythe/Bat3 Antibody

Rabbit Polyclonal
Code

100-401-B63
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western Blot showing detection of mouse Scythe/BAT3 by Rockland's anti-Mouse Scythe/BAT3 antibody in various tissues of mouse WT and Scythe knockout (-/-) embryos.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Human Leukocyte Antigen-B-Associated Transcript 3, HLA-B-associated transcript 3, BAT3, Large proline-rich protein BAT3

Scythe/Bat3 Antibody Properties

Description
Anti-Mouse Scythe/BAT3 (RABBIT) Antibody - 100-401-B63
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human, rat, mouse
Clonality
Polyclonal
Application
ELISA : User Optimized
Western Blot : 1:5,000 to 1:8000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BAT3
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Lot#  

Scythe/Bat3 Antibody Description

Background
Scythe protein (also known as BAT3 and HLA-B-associated transcript 3) is an apoptotic regulator that is highly conserved in eukaryotes and contains a ubiquitin-like domain near its N-terminus. It binds Reaper, a potent apoptotic inducer, as well as Hid and Grim. Scythe and Reaper are thought to signal apoptosis, in part through regulating the folding and activity of apoptotic signaling molecules. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis. It interacts with transforming growth factor-beta (TGF-beta) receptors and enhances TGF-beta1-induced type I collagen expression in mesangial cells. With SET1A, Scythe forms a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. Scythe is essential for p300-mediated acetylation of p53. Scythe/BAT3 is identified as a critical regulator of Hsp70-2 in spermatogenesis, thereby providing a possible molecular target in idiopathic male infertility. The human Scythe/BAT3 ortholog in rodents is predominantly and developmentally expressed in testis.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/BAT3.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approximately 200kDa in size corresponding to Scythe/BAT3 by western blotting in the appropriate cell lysate or extract. In western blot analysis of endogenous protein, multiple bands of Scythe/BAT3 may appear corresponding to variable amount of splice isoforms present.
Purity/Specifity
This product was prepared from monospecific antiserum by addition of Sodium azide to 0.01%. The antibody detects endogenous Scythe/BAT3 in cell lysates.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Fabienne Desmots, Helen R. Russell, Youngsoo Lee, Kelli Boyd, and Peter J. McKinnon (2005). The Reaper-Binding Protein Scythe Modulates Apoptosis and Proliferation during Mammalian Development. Molecular and Cellular Biology, December 2005, p. 10329-10337, Vol. 25, No. 23.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
200-401-A34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-268

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse, rat
Catalog Number
600-401-412

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, Xenopus
Catalog Number
600-401-964

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry