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Primary Antibodies > Ubiquitin and UBL Antibodies

Cdc20 Antibody

Rabbit Polyclonal

Code

100-401-A17

Size

100 µL

Price

$294.00

Availability

Ships next business day.

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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.

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Synonyms: CDC20 cell division cycle 20 homolog antibody, CDC20A antibody, Cell division cycle 20 antibody, Cell division cycle 20 homolog antibody, Cell division cycle protein 20 homolog antibody, fizzy antibody, MGC102824 antibody, p55CDC antibody

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Cdc20 Antibody Properties

Description

Anti-CDC20 (fizzy) (C-terminal specific) [RABBIT) Antibody - 100-401-A17

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:1,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

Antiserum

Label

Unconjugated

Concentration

85 mg/mL by Refractometry

Buffer

None

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

CDC20

Cdc20 Antibody Description

Background

CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G₁/S, protein level peaks in M phase and protein is abruptly degraded at M/G₁ transition.

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This antibody reacts with human CDC20 (fizzy) by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa band corresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressing CDC20 can be used as a positive control. Researchers should determine optimal titers for other applications.

Purity/Specifity

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42. Song,M.S., Song,S.J., Ayad,N.G., Chang,J.S., Lee,J.H., Hong,H.K., Lee,H., Choi,N., Kim,J., Kim,H., Kim,J.W., Choi,E.J., Kirschner,M.W. and Lim,D.S. (2004) The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex. Nat. Cell Biol. 6 (2), 129-137. Zur,A. and Brandeis,M. (2002) Timing of APC/C substrate degradation is determined by fzy/fzr specificity of destruction boxes. EMBO J. 21 (17), 4500-4510.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra