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Primary Antibodies  >  Neuroscience Antibodies
 

CaM Kinase IV Antibody

Rabbit Polyclonal
Code

100-401-883
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's Anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations:  lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate.  Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively).  Approximately 35 µg of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.  CaM Kinase IV was similarly detected on lysates from mouse brain (not shown).  After blocking the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
Rockland's Anti-CAMK4 antibody was diluted 1:500 to detect CAMK4 in human brain cortex tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Synonyms: CAM kinase 4 antibody, CAM kinase GR antibody, CAM kinase IV antibody, CaMK4 antibody, CaMKGR antibody, Brain Ca(2+) calmodulin dependent protein kinase type IV antibody, Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody

CaM Kinase IV Antibody Properties

Description
Anti-CaM Kinase IV (RABBIT) Antibody - 100-401-883
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, dog, bovine, chimpanzee
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
70 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CaMK IV
Download CofA
Lot#  

CaM Kinase IV Antibody Description

Background
CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18.   CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory.  CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2.  In addition autophosphorylation of the N-terminus is required for full activation.  Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state.  Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A.  CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix.  CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.
Immunogen
This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 305-323 of Human CaM Kinase IV protein.
Immunogen Type
Peptide
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
This antiserum has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 52 kDa in size corresponding to CaM Kinase IV by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This antiserum is directed against human CaM Kinase IV protein.  The product was delipidated, defibrinated followed by buffering and clarification.  A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, dog, bovine and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CaM Kinase IV protein from zebrafish, chicken and frog may occur as this sequence only varies by one or two amino acid residues (~89% homology). Cross reactivity with CaM Kinase IV homologues from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Kitani,T., Okuno,S. and Fujisawa,H. (1994) cDNA cloning and expression of human calmodulin-dependent protein kinase IV. J. Biochem. 115 (4), 637-640. Bland,M.M., Monroe,R.S. and Ohmstede,C.A. (1994) The cDNA sequence and characterization of the Ca2+/calmodulin-dependent protein kinase-Gr from human brain and thymus. Gene 142 (2), 191-197. Mosialos,G., Hanissian,S.H., Jawahar,S., Vara,L., Kieff,E. and Chatila,T.A. (1994) A Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, expressed after transformation of primary human B lymphocytes by Epstein-Barr virus (EBV) is induced by the EBV oncogene LMP1. J. Virol. 68 (3), 1697-1705.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF, IP
Reacts With
mouse
Catalog Number
100-401-962

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Rabbit
Catalog Number
211-1102

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat, dog, orangutan
Catalog Number
600-401-873

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry